Rapid Identification of Microorganisms Isolated from Pickled Vegetables by MALDI-TOF Mass Spectrometry

Zhi Lei Tan; Yu Qiao Wei; Shuang Liang; Ran Zhang; Miao Liu; Shi Ru Jia

doi:10.4028/www.scientific.net/AMR.712-715.494

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 712-715Rapid Identification of Microorganisms Isolated...

Rapid Identification of Microorganisms Isolated from Pickled Vegetables by MALDI-TOF Mass Spectrometry

Abstract:

Matrix-assisted laser desorption/ionization time-off light mass spectrometry (MALDI-TOF MS) is increasingly used as a microbial diagnostic method for species identification of pathogens. However, MALDI-TOF MS identification of food bacteria was seldom reported. Ten strains isolated from different pickled vegetables were rapid identified by MALDI-TOF MS. The results of MALDI-TOF MS were confirmed by 16S rDNA sequencing method. Different score values in MALDI-TOF MS revealed nine Leuconostoc mesenteroides and one Staphylococcus. Identification success at the species and genus levels was 90% (9/10) and 100% (10/10), respectively.16S rDNA sequencing results showed that nine stains were identified as Leuconostoc mesenteroides and one was Staphylococcus saprophyticus. Results obtained demonstrate that MALDI-TOFMS is a promising method for discriminating and identifying food bacteria.

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Advanced Materials Research (Volumes 712-715)

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494-497

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.712-715.494

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Online since:

June 2013

Authors:

Zhi Lei Tan, Yu Qiao Wei, Shuang Liang, Ran Zhang, Miao Liu, Shi Ru Jia

Keywords:

16S rRNA, Biotyper, MALDI-TOF MS, Rapid Microorganism Identification

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