Core-Shell Structured Fe₃O₄/PPy Microspheres with High Magnetization for Purification of Plasmid DNA

Abstract:

Amino group-functionalized magnetic particles have wide applications in enzyme immobilization, DNA extraction, drug delivery, water purification, catalysis, and sensor. In this paper, Fe3O4/PPy microspheres with a well-defined coreshell structure have been prepared through an interfacial polymerization approach without surfactant. The magnetic composite spheres were characterized with XRD, FTIR, SEM, TEM, and magnetometry techniques, and further tested as the adsorbent to isolate plasmid DNA from Escherichia coli (E. coli) DH5α cells. The magnetic separation yields high-quality plasmid DNA in satisfying productivity as compared to the conventional phenolchloroform extraction.

• Periodical: Advanced Materials Research (Volume 716)
• Pages: 314-319
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.716.314
• Online since: July 2013
• Authors: Hai Hui Jiang, Yan Zhou, Xiao Yun Han, Xin Cheng Chen, Yun Hua Hou, Li Gang Gai
• Keywords: Escherichia сoli Cells, Fe₃O₄/PPy Microspheres, Magnetic Separation

References

[1] J. Garcia, Y. Zhang, H. Taylor, O. Cespedes, M.E. Webb and D. Zhou: Nanoscale 3 (2011) 3721–3730.

[2] Y. Cui, Y. Li, Y. Yang, X. Liu, L. Lei, L. Zhou and F. Pan: J. Biotechnol. 150 (2010) 171–174.

[3] W. Cao, C.J. Easley, J.P. Ferrance and J.P. Landers: Anal. Chem. 78 (2006) 7222–7228.

[4] J.L. Arias, H. Reddy and P. Couvreur: J. Mater. Chem. 22 (2012) 7622–7632.

[5] Y. Wang, B. Zou, T. Gao, X. Wu, S. Lou and S. Zhou: J. Mater. Chem. 22 (2012) 9034‒9040.

[6] G.S. Lai, H.L. Zhang and D.Y. Han: Sens. Actuators B 129 (2008) 497–503.

[7] J. Deng, X. Ding, W. Zhang, Y. Peng, J. Wang, X. Long, P. Li and A.S.C. Chan: Polymer 43 (2002) 2179–2184.

[8] L. Gai, X. Han, Y. Hou, J. Chen, H. Jiang and X. Chen: Dalton T. 42 (2012) 1820‒1826.

[9] D.H. Park, J.M. Oh, Y.G. Shul and J.H. Choy: J. Nanosci. Nanotechnol. 8 (2008) 5014‒5017.

[10] H. Zhang, X. Zhong, J.J. Xu and H.Y. Chen: Langmuir 24 (2008) 13748–13752.

[11] X. Lu, H. Mao and W. Zhang: Polym. Compos. 30 (2009) 847–854.

[12] S. Xuan, Y.X. J. Wang, K.C.F. Leung and K. Shu: J. Phys. Chem. C 112 (2008) 18804–18809.

[13] H. Deng, X.L. Li, Q. Peng, X. Wang, J.P. Chen and Y.D. Li: Angew. Chem. Int. Ed. 44 (2005) 2782–2785.

[14] X. Xu, C. Deng, M. Gao, W. Yu, P. Yang and X. Zhang: Adv. Mater. 18 (2006) 3289–3293.

[15] C.L. Chiang, C.S. Sung, T.F. Wu, C.Y. Chen and S.Y. Hsu: J. Chromotogr. B 822 (2005) 54‒60.

[16] K. Tao, H.J. Dou and K. Sun: Chem. Mater. 18 (2006) 5273‒5278.

[17] L. Gai, G. Du, Z. Zuo, Y. Wang, D. Liu and H. Liu: J. Phys. Chem. C 113 (2009) 7610‒7615.

[18] K. Yoshikawa: Adv. Drug Deliv. Rev. 52 (2001) 235–244.

[19] K.A. Melzak, C.S. Sherwood, R.F.B. Turner and C.A. Haynes: J. Colloid Interf. Sci. 181 (1996) 635–644.

[20] T. Tanaka, R. Sakai, R. Kobayashi, K. Hatakeyama and T. Matsunaga: Langmuir 23 (2009) 2956–2961.

[21] L. Gai, Z. Li, Y. Hou, H. Jiang, X. Han and W. Ma: J. Phys. D: Appl. Phys. 43 (2010) 445001.