Self-Assembling Poly(ethylene glycol)/Poly-γ-glutamic Acid Nanoparticles for Targeted Drug Delivery and Plasmid DNA
These stable self-assembled nanoparticles were characterized by dynamic light scattering, transmission electron microscopy, and atomic force microscopy,which demonstrated that the nanosystem consists of spherical particles with a smooth surface both in aqueous environment and in dried state. Toxicity measurements showed that the composition is nontoxic when tested either on cell cultures or in animal feeding experiments. To evaluate the potential of the nanosystem for intracellular drug delivery and gene, the nanoparticles were fluorescently labeled and folic acid was attached as a cancer cell-specific targeting moiety. The quantitative data obtained by digital processing of the intensity of green color of each pixel in the pictures inside the cell boundaries and total intensity of fluorescence inside the cells showed thattargeted particles internalized into the cells significantly faster and the total accumulation of these particles was substantially higher in the cancer cells.
Y. Ge et al., "Self-Assembling Poly(ethylene glycol)/Poly-γ-glutamic Acid Nanoparticles for Targeted Drug Delivery and Plasmid DNA", Advanced Materials Research, Vol. 721, pp. 105-108, 2013