Optimization of the SPME-GC-MS Techniques for Analyzing Headspace Volatiles in Shrimp Head of P. borealis

Abstract:

To investigate the headspace volatiles in shrimp head P. borealis, optimized conditions were evaluated for solid-phase microextraction coupled to gas chromatography-mass spectrometry method. SPME fibers of 100-μm polydimetylsiloxane, 65-μm polydimetylsiloxane-divinylbenzene, 75-μm carboxen-polydimetylsiloxane, and 50/30-μm divinylbenzene-carboxen-polydimetylsiloxane (DVB/CAR/PDMS) were compared. The influence of several parameters such as type of fibers, volume of sample, extraction time, extraction temperature, and state of samples was systematically investigated. Suitable reproducibility and sensitivity were achieved by combining DVB/CAR/PDMS of 50/30-μm thickness as the liquid phase of SPME, 1.5 g of shrimp head lyophilized powder, 40 ^OC of isolation temperature and 40 min of isolation time. A total of 62 volatile compounds have been confirm, including hydrocarbons, ketones, alcohols, aldehydes, esters, nitrogen-containing compounds and sulfur-containing compounds. 3,5-octadien-2-one (E,Z), 3,5-octadien-2-one (E,E), 1-penten-3-one, 6-octen-2-one, ethyl acetate and alcohols such as 1-penten-3-ol, 2-penten-1-ol and 1-octen-3-ol were predicted as key compounds for SH flavor.

• Periodical: Advanced Materials Research (Volumes 726-731)
• Pages: 416-419
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.726-731.416
• Online since: August 2013
• Authors: Wan Cui Xie, Xin Lian, Xi Hong Yang, Ya Dong Yang, Dan Hui Mao
• Keywords: Gc-Ms, Optimization, Shrimp Head (SH) of P. borealis, Solid-Phase Micro-Extraction (SPME), Volatile Compounds

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