Study on Fluorescent Staining of Bacteria for Total Direct Counts in Polycyclic Aromatic Hydrocarbons (PAHs) Contaminated Soil with 5-(4,6-DichloroTriazin-2-yl) Amino Fluorescein (DTAF)

Dan Zhang; Ai Zhong Ding; Lin Jiang; Yue Hua Cai; Li Na Zhang

doi:10.4028/www.scientific.net/AMR.726-731.452

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Study on Fluorescent Staining of Bacteria for Total Direct Counts in Polycyclic Aromatic Hydrocarbons (PAHs) Contaminated Soil with 5-(4,6-DichloroTriazin-2-yl) Amino Fluorescein (DTAF)
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 726-731Study on Fluorescent Staining of Bacteria for...

Study on Fluorescent Staining of Bacteria for Total Direct Counts in Polycyclic Aromatic Hydrocarbons (PAHs) Contaminated Soil with 5-(4,6-DichloroTriazin-2-yl) Amino Fluorescein (DTAF)

Abstract:

Using DTAF fluorescent staining technique, the number of bacteria and the distributions of ΣPAHs in two different drilling vertical depths of a field contaminated by PAHs are counted and analyzed. The result showed that the distributions of the number of bacteria and the ΣPAHs in the vertical depth have obvious corresponding relations, and the higher the ΣPAHs is, the more the number of bacteria would be.

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Periodical:

Advanced Materials Research (Volumes 726-731)

Pages:

452-455

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.726-731.452

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Online since:

August 2013

Authors:

Dan Zhang, Ai Zhong Ding, Lin Jiang, Yue Hua Cai, Li Na Zhang

Keywords:

Bacteria for Total Direct Counts, DTAF Fluorescent Staining Technique, Polycyclic Aromatic Hydrocarbons (PAHs), Soil

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References

[1] P.C. Brookes: Biology and Fertility of Soils. Vol. 19 (1995), pp.269-279

Google Scholar

[2] B. Wick, R.F. Kuhne and P.L.G. Vlek: Plant Soil. Vol. 202 (1998), pp.97-107

Google Scholar

[3] P.K. Gupta: Methods in environmental analysis: water, soil and air(2nd). (India Jodhpur, 2007).

Google Scholar

[4] K. Kaufmann, M. Christophersen, A. Buttler, et al: FEMS Microbiology Ecology. Vol. 48(3) (2004), pp.387-399

Google Scholar

[5] H.M. Shapiro: Journal of Microbiological Methods. Vol. 42(1) (2000), pp.3-16

Google Scholar

[6] Z.X. Zeng, S.M. Clark, R.A Mathies,et al: Analytical Biochemistry, Vol.252(1)(1997),pp.110-114

Google Scholar

[7] M.V. Sizova, J.A. Izquierdo, N.S. Panikov, et al: Applied and Environmental Microbiology. Vol. 77 (2011), pp.2282-2291

Google Scholar

[8] Y.J. Zhang, S.Q. Zhu, R. Xiao,et al: Research of Environmental Sciences, Vol. 20 (2007), pp.7-11

Google Scholar

[9] J. Bloem, A. Vos: Molecular Microbial Ecology Manual (2nd), (2004), pp.861-874

Google Scholar

[10] G.B Davis, C.D Johnston, B.M Patterson, et al. Ground Water Monitoring & Remediation. Vol. 18(2) (1998), pp.123-132.

Google Scholar