The Recovery Effect of the Translationally Controlled Tumor Protein in 2-Hydroxy-Ethyl Methacrylate-Treated Human Dental Pulp Cells

Anchana Kongsaengkaeo; Wilaiwan Chotigeat; Ureporn Kedjarune-Leggat

doi:10.4028/www.scientific.net/AMR.747.153

Paper Titles

Behaviour of Lipid-Based Formulations Containing Nifedipine in Aqueous Media as Observed by Small Angle X-Ray Scattering
p.139

Preparation of Solid Self-Emulsifying Drug Delivery System of Manidipine Hydrochloride
p.143

PEG-Glu-PEI-Dexamethasone Conjugates: Synthesis, Characterization and In Vitro Gene Transfer Properties
p.147

The Influence of Amount of Succinic Anhydride in Chain Extension Reaction on Increasing Molecular Weight of Poly(lactic acid)
p.148

The Recovery Effect of the Translationally Controlled Tumor Protein in 2-Hydroxy-Ethyl Methacrylate-Treated Human Dental Pulp Cells
p.153

Influence of Bagasse Carboxymethyl Cellulose Addition on the Thermal and Mechanical Properties of PLA Composites
p.157

Dissolution Improvement of Itraconazole by a Nanoparticulate System Containing Lecithin-Pectin Complexes
p.162

Fabrication and Antifungal Activity of Cellulose Acetate-Based Fibers Encapsulating Natural Neem Seed Oil
p.166

Preparation of 3DP Hydroxyapatite/Polycaprolactone Composite by a Novel Sequential Infiltration Technique
p.170

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 747The Recovery Effect of the Translationally...

The Recovery Effect of the Translationally Controlled Tumor Protein in 2-Hydroxy-Ethyl Methacrylate-Treated Human Dental Pulp Cells

Abstract:

2-Hydroxy-ethyl methacrylate (HEMA) is a major monomer released from resin-base dental restorative materials. This study examined the recovery effect of the Translationally Controlled Tumor Protein (TCTP) in human dental pulp cells (HDPCs) after exposed to HEMA. TCTP from banana prawn (Penaeus merguiensis) was cloned and the protein was purified. A real-time cell analyzer was used to evaluate cell survival. The cell suspensions were seeded into an E-plate 96 at 8,000 cells/ well. After 24 hours, HDPCs were treated with 8 mM HEMA mixed in culture medium (alpha modified Eagles medium, α-MEM) for 1 hour before the medium supplemented with TCTP at 0, 100 ng/ml, 1 μg/ml, 10 μg/ml was replaced and left for 23 hours. After that, cells were fed with fresh medium for 72 hours. The cell indexes were monitored every 15 minutes and the 1-way analysis of variance (ANOVA) was used for statistical analysis. Real-time xCELLigence impedance analysis indicated that the cell indexes reached to 0 after treated with HEMA for 1 hour. TCTP at 1 μg/ml was significantly (P < 0.05) increased the cell index of HEME-treated HDPCs from 0 to 0.02 and 0.04 after TCTP exposure for 1 hour and 23 hours, respectively. It was suggested that TCTP has an ability to recover the cytotoxic effect of HEMA-treated pulp cells.

You might also be interested in these eBooks

Multi-Functional Materials and Structures IV

View Preview

Info:

Periodical:

Advanced Materials Research (Volume 747)

Pages:

153-156

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.747.153

Citation:

Cite this paper

Online since:

August 2013

Authors:

Anchana Kongsaengkaeo, Wilaiwan Chotigeat, Ureporn Kedjarune-Leggat

Keywords:

2-Hydroxy-Ethyl Methacrylate, Human Dental Pulp Cells, Translationally Controlled Tumor Protein

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] W. Geurtsen, Biocompatibility of resin-modified filling materials, Crit Rev Oral Biol Med. 11 (2000) 333–355.

Google Scholar

[2] H. Schweikl, G. Spagnuolo, G. Schmalz, Genetic and cellular toxicology of dental resin monomers, J. Dent Res. 85 (2006) 870–877.

DOI: 10.1177/154405910608501001

Google Scholar

[3] W. Geurtsen, W. Spahl, K. Muller, G. Leyhausen, Aqueous extracts from dentin adhesives contain cytotoxic chemicals, J. Biomed Mater Res. 48 (1999) 772–777.

DOI: 10.1002/(sici)1097-4636(1999)48:6<772::aid-jbm2>3.0.co;2-x

Google Scholar

[4] S. Bouillaguet, J.C. Wataha, C.T. Hanks, B. Ciucchi, J. Holz, In vitro cytotoxicity and dentin permeability of HEMA, J. Endod. 22 (1996) 244–248.

DOI: 10.1016/s0099-2399(06)80141-x

Google Scholar

[5] G. Ciapetti, D. Granchi, E. Cenni, L. Savarino, D. Cavedagna, A. Pizzoferrato, Cytotoxic effect of bone cements in HL-60 cells: distinction between apoptosis and necrosis, J. Biomed Mater Res. 52 (2000) 338-345.

DOI: 10.1002/1097-4636(200011)52:2<338::aid-jbm13>3.0.co;2-l

Google Scholar

[6] U.I. Walther, I.I. Siagian, S.C. Walther, F.X. Reichl, R. Hickel, Antioxidative vitamins decrease cytotoxicity of HEMA and TEGDMA in cultured cell lines, Arch Oral Biol. 49 (2004) 125–131.

DOI: 10.1016/j.archoralbio.2003.08.008

Google Scholar

[7] G. Spagnuolo, V. D'Anto, C. Cosentino, G. Schmalz, H. Schweikl, S. Rengo, Effect of N-acetyl-L-cysteine on ROS production and cell death caused by HEMA in human primary gingival fibroblasts, Biomaterials. 27 (2006) 1803–1809.

DOI: 10.1016/j.biomaterials.2005.10.022

Google Scholar

[8] N. Wanachottrakul, W. Chotigeat, U. Kedjarune-Leggat, Translationally controlled tumor protein against apoptosis from 2-hydroxy-ethyl methacrylate in human dental pulp cells, J. Mater Sci Mater Med. 22 (2011) 1479–1487.

DOI: 10.1007/s10856-011-4328-1

Google Scholar

[9] E. Urcan, U. Haertel, M. Styllou, R. Hickel, H. Scherthan, F.X. Reichl, Real-time xCELLigence impedance analysis of the cytotoxicity of dental composite components on human gingival fibroblasts, Dent Mater. 26 (2010) 51-58.

DOI: 10.1016/j.dental.2009.08.007

Google Scholar

[10] Roche Diagnostics, Introduction of the RTCA SP instrument, in: RTCA SP instrument operator's manual. Indianapolis: ACEA Biosciences Inc., San Diego, 2008, pp.14-16.

Google Scholar

[11] W. Loongyai, A. Phongdara, C. Wilaiwan, Cloning and expression of a TCTP homolog from the ovaries of banana prawn, Mar Biol. 150 (2007) 455–462.

DOI: 10.1007/s00227-006-0375-4

Google Scholar