Analysis of Genetic Subtypes and Distribution of Hantavirus in Jilin Area

Abstract:

To investigate the subtypes and distribution of hantavirus in Jilin area. Rattus norvegicus were collected from the main endemic areas of Jilin area and hantavirus in the lung were identified by indirect immunofluorescence assay. M segments of positive samples were partially amplified with Nested RT-PCR and sequenced. Phylogenetic analysis was used for genotyping. All hantaviruses from R. norvegicus were Seoul virus in Jilin area. Phylogenetic analysis of M segments showed that Seoul hantaviruses carried by R. norvegicus in Jilin city were the S3 subtype,and there was no HTNV and PUUV found. The results showed that the subtype of Seoul hantaviruses in Jilin area was S3.

• Periodical: Advanced Materials Research (Volume 749)
• Pages: 362-365
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.749.362
• Online since: August 2013
• Authors: Chen Zhao, Yang Zhao, Zhong Hai Yuan, Ke Xin Sun, Yan Li
• Keywords: Hantavirus, HFRS, Phylogenetic Analysis, Rattus norvegicus , Subtypes

References

[1] Zhao, C., Y. Sun, Y. Zhao, et al. Immunogenicity of a multi-epitope DNA vaccine against hantavirus. Hum Vaccin Immunother, 2012. 8(2): pp.208-15.

[2] Schmaljohn, C.,B. Hjelle. Hantaviruses: a global disease problem. Emerg Infect Dis, 1997. 3(2): pp.95-104.

DOI: 10.3201/eid0302.970202

[3] HUANG Biao, PENG Yue-hua, ZHANG Wei-han, et al. Analysis on Epidemic Situation of Hemorrhagic Fever with Renal Syndrome in Jilin Province During the Period of 2005～2006. Practical Preventive Medicine, 2008(02): pp.413-414.

[4] WU Yan-ping, HUANG-biao, HU Guang-wei, et al. Analysis on epidemiologic features of HFRS in Jilin Province. Chinese Journal of Control of Endemic Disease, 2004(03): pp.164-165.

[5] SUN Chen qun, CHEN Lu fei, WU Yu hua, et al. Molecular biological evidence of Seoul virus firstly isolated in the Northeast China (Heilongjiang province): Complete nucleotide sequence analysis of the S genome segment of hantavirus Pf26 strain, Chinese Journal of Disease Control and Prevention, 2000(04): pp.313-316.

[6] WANG Shi wen, HANG Chang shou, WANG Hua, et al. Genotype and Clade Distribution of Hantaviruses in China. Chinese Journal of Virology, 2002, 18: 211-216.

[7] WU Dong-lin, WANG Hui, SHEN Bo, et al. RT-PCR Detection and Sequence Analysis of Puumala Virus in Jilin Province. Chinese Journal of Public Health Engineering, 2008(05): pp.259-261.

[8] TANG Li-hua, ZHANG Quan-fu, XIU Mei-hong, et al. Identification of a New Puumala Hantavirus Subtype in Rodents from China. Chinese Journal of Virology, 2007(04): pp.320-325.

[9] Tamura, K., D. Peterson, N. Peterson, et al., MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 2011. 28(10): pp.2731-9.

DOI: 10.1093/molbev/msr121

[10] Schmaljohn, C., Vaccines for hantaviruses. Vaccine, 2009. 27 Suppl 4: p. D61-4.

DOI: 10.1016/j.vaccine.2009.07.096

[11] TU Yu-ping, YANG Xiao-ping. PUUV Epidemic Outbreak in Sweden. Disease Surveillance, 2008(06): p.396.

[12] YAN Yan-zhen, YAO Lai-shun, HU Guang-wei, et al. Genetic Subtypes and Distribution of Seoul Virus in Jilin. Chinese Journal of Vector Biology and Control, 2006(04): pp.324-326.

[13] Chen Huaxin, Qiu Fuxi, Zhao Xiouqin, et al. Characteristics of the Distribution of Epidemic Season of Hemorrhagic Fever with Renal Syndrome in Different Regions and Different Years in China. Chinese Journal of Experimental and Clinical Virology, 1994(03): pp.197-203.