A Technique for Agrobacterium-Mediated Transformation via Germinating Seeds of Soybean

Abstract:

The plasmid (with resistant the herbicide glyphosate gene EPSPS and glufosinate resistance screening gene Bar) was transferred into soybean [Glycine max (L.) Merr.] by agrobacterium-mediated transformation, and the Bar gene as the selectable marker coupled with glufosinate as a selective agent is described. This method used for transformation requires without tissue culture steps. Soybean seeds were germinated for 1 day, plumule, cotyledonary node, and adjacent regions were wounded with the tip. The wounded half-seeds were inoculated with agrobacterium tumefaciens cells harboring a recombinant DNA that contained the EPSPS and Bar genes. Polymerase chain reaction and two herbicides screened proved that the EPSPS gene has been transferred into soybean successfully; the transformation rate of soybean about was 0.37%.

• Periodical: Advanced Materials Research (Volume 749)
• Pages: 413-417
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.749.413
• Online since: August 2013
• Authors: Zhi Min Liu, He Wen Xiong, Hao Xie, Yu Tao Qin, Yan Ran Zhao, Bei Guo
• Keywords: Agrobacterium, Germinating Seed, Soybean [Glycine Max (L.) Merr.], Transformation

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