A New Electrochemical Aptasensor for Protein Detection Based on Target Protein-Induced Strand Displacement

Abstract:

A sensitive electrochemical aptasensor for detection of thrombin based on target protein-induced strand displacement is presented. For this proposed aptasensor, dsDNA which is from immobilized probe DNA (IP) which has a hybridized with aptamer(Apt) initially was immobilized on the Au electrode through thiol group of IP，while a DNA labeled with PdS nanoparticles was used as a detection probe (DP-PdS). When Au electrode immersed into solution which contain target protein and DP-PdS, the aptamer preferred to form G-quarter structure with the present target protein and dsDNA was released into immobilized probe DNA (IP) which could be hybridized with DP-PdS. After dissolving PdS from electrode, a mercury-film electrode was used for electrochemical detection of these Pd2+ ions which offered excellent electrochemical signal transduction. Detection of thrombin was specific without being affected by the coexistence of other proteins such as BSA and lysozyme, and as low as 2.3×10-11 mol/L of thrombin was detected.

• Periodical: Advanced Materials Research (Volumes 753-755)
• Pages: 2113-2116
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.753-755.2113
• Online since: August 2013
• Authors: Han Feng Cui, Hao Fan, Yan Lin
• Keywords: Displacement, DNA Hybridization, Electrochemical Aptasensor, PdS Nanoparticles

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