The Expression of HIF-L a and VEGF in Endochondral Ossification Processes

Abstract:

Objective To investigate the expression of HIF-la in entochondrostosis,and to study its role in the process of vascular intrusion.Methods The morphology change in rats femoral bone development were observed using HE staining method.The protein expression Of HIF-Ia and VEGF in femoral bone was detected by immunohistochemistry,and the mRNA expression of HIF-Ia and VEGF was detected by reverse transcription-polymerase chain reaction (RT-PCR) method.Results: Many cartilage cells were seen in femoral bone at the 1st day after the birth;many vessels began to invade at the 7th dayfat the 13th day,the cartilage matrix began to calcification,vessels and cartilage cells were reduced.The protein expression of HIF-la and VEGF was increased at the 7th daywhile decreased at the 13th day.The mRNA expression of HIF-la and VEGF went up to the maximum at 7th day the time,and then decreased.Conclusion During femoral bone development,hypoxia may induce HIF-I expression,then activates its downstream gene VEGF expression,which would induce the intrusion of exogenous vascular.Promote endochondral osteogenesis.

• Periodical: Advanced Materials Research (Volume 773)
• Pages: 342-346
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.773.342
• Online since: September 2013
• Authors: Wang Song, Jin Xing
• Keywords: Bone Development, HIF-1a, Hyproxia, VEGF

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