Fingerprint Analysis of Polygonatum odoratum (Mill.) Druce Using High-Performance Liquid Chromatographic Method with Diode-Array Detection

Guang Sheng Xi

doi:10.4028/www.scientific.net/AMR.781-784.1199

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 781-784Fingerprint Analysis of Polygonatum odoratum...

Fingerprint Analysis of Polygonatum odoratum (Mill.) Druce Using High-Performance Liquid Chromatographic Method with Diode-Array Detection

Abstract:

The fingerprints analysis of different lines of Polygonatum odoratum (Mill.) Druce were established by high-performance liquid chromatographic with diode-array detection (HPLC/DAD). The analyses were performed on ZORBAX Eclipse XDB-C₁₈ (4.6×250 mm, 5 μm) column using a mobile phase (aqueous 0.05% formic acid-acetonitrile) under gradient conditions. The flow rate was 1.0 ml/min. The detecting wavelength was set at 210 nm. HPLC fingerprints of two lines were little different. The similarity of the same line was greater than 0.9, and the average similarity of the different lines was 0.794. This method could be suitable for the line identification and quality control of Polygonatum odoratum (Mill.) Druce.

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Periodical:

Advanced Materials Research (Volumes 781-784)

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1199-1202

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.781-784.1199

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Online since:

September 2013

Authors:

Guang Sheng Xi

Keywords:

Fingerprint, High-Performance Liquid Chromatographic Method, Polygonatum Odoratum (Mill.) Druce

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