Study of Affinity Interaction of Paeonol and BSA by Vacancy Affinity Capillary Electrophoresis

Article Preview

Abstract:

In this paper, paeonol was studied systemtically. At first, the affinity interaction of paeonol with bovine serum albumin (BSA) was investigated through capillary electrophoresis approach. The association constant of paeonol and BSA was obtained by the vacancy affinity capillary electrophoresis method (VACE). The second, the contents of paeonol were determinede in liuweidihuang pill. In this paper, the new studied method and basic data were offered for further investigating the pharmacology and drug efficacy of paeonol. Meanwhile, the method described would be particularly useful in the quality control of traditional Chinese medicines-liuweidihuang pill.

You might also be interested in these eBooks

Info:

Periodical:

Advanced Materials Research (Volumes 781-784)

Pages:

1209-1214

Citation:

Online since:

September 2013

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2013 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] N. H. H. Heegaard and R. T. Kennedy, Electrophoresis 1999, 20, 3122.

Google Scholar

[2] M. H. A. Bush, L. B. Carels, H. F. M. Boelens, J. C. Kraak, H. Poppe, J. Chromatogr. A, 1997, 777, 311.

Google Scholar

[3] John Sowell, Kimberly A. Agnew-Heard, J. Christian Mason, Charles Mama, Lucjan Strekowski, Gabor Patonay, J. Chromatogr. B, 2001, 755, 91.

Google Scholar

[4] Mingfang Hong, Aaron Cassely, Yehia Mechref, Milos V. Novotny, J. Chromatogr. B, 2001, 752, 207.

Google Scholar

[5] M. H. A. Busch, H. F. M. Boelens, J. C. Kraak, H. Poppe, J. Chromatogr. A, 1997, 775, 313.

Google Scholar

[6] F. B. Erim, J. C. Kraak, J. Chromatogr. B, 1998, 710, 205.

Google Scholar

[7] Ying Sun Ning Fang David D. Y. Chen Electrophoresis, 2008, 29, 3333–3341.

Google Scholar

[8] Xiao Hong, Yu Guojun, Jin Ronglan. Chinese J. of Pharmaceutical analysis, 1989, 9, 225.

Google Scholar

[9] Wang Xianyang, Wang Xin, Chinese J. of Modern Pharmaceutical, 1998, 15, 17.

Google Scholar

[10] Haixingliu, Aimin Yu, Fengqin Liu, Yuhua Shi, Likun Han, Yunfa Chen. Chiral separation of cefadroxil by capillary electrochromatography, J. of Pharmaceutical and Biomedical Analysis, 2006, 41(4), 1376-1379.

DOI: 10.1016/j.jpba.2006.02.032

Google Scholar

[11] Haixing Liu Yuhua Shi Dexian Wang Gengliang Yang Aimin Yu Hanqi Zhang. MECC determination of oleanolic acid and ursolic acid isomers in ligustrum lucidum ait. J. of Pharmaceutical and Biomedical Analysis, 2003, 32, 479-485.

DOI: 10.1016/s0731-7085(03)00235-8

Google Scholar