Effects of Polychlorinated Biphenyl (PCB101) and its Hydroxylated Metabolites on Proliferation of MCF-7 Human Breast Cancer Cells

Abstract:

Polychlorinated biphenyls and their hydroxylated metabolites become more and more concerned for their endocrine disruption. At the presented study, experiment was designed to obtain the disruption effect of PCB101 and its hydroxylated metabolites . The proliferation of MCF-7 human breast cancer cells was detected at different exposed concentration and time period. MCF-7 cells were cultured with the concentration of PCB101 and twor of its hydroxylated metabolites at 5×10^-95×10^-85×10^-75×10^-6mol/L. And the concentrations of 17β-Estradiol (E2) were2×10^-92×10^-82×10^-72×10^-6mol/L respectively. And the proliferation rate was detected after 24h,48h, 72h, 96h exposure.Ethanol was used as solvent control.The results indicated that PCB101 and its hydroxylated metabolites had no effect on MCF-7 cell proliferation at designed concentrations and exposure time.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 927-931
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.927
• Online since: September 2013
• Authors: Yong Fu Shi, Zhou Luo Ou, You Qiong Cai, Xiao Li Gu, Dong Mei Huang, Ting Zhang, Yuan Wang, Hui Juan Yu
• Keywords: Hydroxylated Polychlorinated Biphenyls, MCF-7, PCB101, Proliferation

References

[1] D. Tan, J B. Zhang J. Environmental pollution &control. Vol. 30, ( 2008), pp.7-20.

[2] H. Allaoua, R. Chicheportiche. European Journal of Pharmacology, Vol. 163, (1989), pp.327-335.

[3] J. Laitner, R. Erben, G.I.V. Klobučar . Toxicology Letters, Vol. 74, (1994), p.45.

[4] L.L. Lu, T. Mamiya, P. Lu, K. Toriumi, A. Mouri, M. Hiramatsu, L.B. Zou, Toshitaka Nabeshima. Behavioural Brain Research. Vol. 220 (2011), pp.132-139.

DOI: 10.1016/j.bbr.2011.01.035

[5] H. J. Chang. Environment and Health. Vol. 19, ( 2002), pp.279-230.

[6] M.N. Abbas, G.A.E. Mostafa and A.M.A. Talanta. Vol . 55, (2001), p.647–656.

[7] A. Oubin, D. Puig, J. Gascon , D. barcelo. Anal. Chim. Acta, Vol. 346 , (1997), p.49–59.

[8] O. Jauregui and M.T. Galceran. Anal. Chim. Acta,  Vol. 340, (1997), p.191–199.

[9] H.C. Hong, H.Y. Zhou, T.G. Luan, C.Y. Lan. Environment International . Vol. 31, (2005), p.643 – 649.

[10] S.J. Young, J.P. Hart, A.A. Downman and D.C. Cowell. Biosens. Bioelectron., Vol. 16 (2001), p.887–894.

[11] A. Mufeed Awawdeh, H. James Harmon. Biosensors and Bioelectronics. 20, (2005), pp.1595-1601.

[12] L. Lin, L. C Liao. J. Forensic Science and Technologe. Vol. 2(2004), pp.10-13.

[13] Z. Q. Huang, H Y Nie, S. H Peng. J. Analytical Chemistry. Vol. 20, ( 1991), pp.38-40.

[14] Z. L Fei. J. Nanjing Normal University Natural Science Edition. Vol. 27, ( 2004), pp.70-73.