Biotransformation of Adiponitrile to 5-cyanovaleramide by Pseudomonas Sp. SY031 Resting Cells

Jing Chen; Yu Ting Huang; Shang Gui Deng; Jian Rong Li

doi:10.4028/www.scientific.net/AMR.791-793.204

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 791-793Biotransformation of Adiponitrile to...

Biotransformation of Adiponitrile to 5-cyanovaleramide by Pseudomonas Sp. SY031 Resting Cells

Abstract:

Resting cells of Pseudomonas sp. SY031 were used for the direct transformation of adiponitrile to 5-cyanovaleramide (5-CVAM), which is an important intermediate of azafenidin, a new kind of herbicide with high efficiency, low toxicity and non-pollution. In this study, reaction conditions for this nitrile hydratase mediated conversion were optimized. It was observed that the maximum conversion of adiponitrile to 5-CVAM was in a solution containing 50 mM phosphate buffer (pH 6.4), 10 mM adiponitrile at 35 °C. 10 mM adiponitrile was completely converted into 5-CVAM and adipamide in shaking flasks with 5-CVAM as the main product (9.004 mM) and adipamide as a low yield (0.896 mM), indicating high regio-selectivity of the nitrile hydratase in Pseudomonas sp. SY031.

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Periodical:

Advanced Materials Research (Volumes 791-793)

Pages:

204-207

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.791-793.204

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Online since:

September 2013

Authors:

Jing Chen, Yu Ting Huang, Shang Gui Deng, Jian Rong Li

Keywords:

5-Cyanovaleramide, Adiponitrile, Biotransformation, Nitrile Hydratase, Pseudomonas Sp. SY031, Regioselective

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References

[1] J. G. Hu, Y. J. Wang, Y. G. Zheng and Y. C. Shen: Enzyme Microb Technol Vol. 41 (2007).

Google Scholar

[2] C. D. Mathew, T. Nagasawa, M. Kobayashi and H. Yamada: Appl Environ Microbiol Vol. 54 (1988), p.1030.

Google Scholar

[3] R. C. Zheng, Y. S. Wang, Z. Q. Liu, L. Y. Xing, Y. G. Zheng and Y. C. Shen: Res Microbiol Vol. 158 (2007), p.258.

Google Scholar

[4] Y. Asano, Y. Tani and H. Yamada: Agric. Biol. Chem. Vol. 44 (1980), p.2251.

Google Scholar

[5] D. Cowan, R. Cramp, R. Pereira, D. Graham and Q. Almatawah: Extremophiles. Vol. 2 (1998), p.207.

Google Scholar

[6] L. Martinkova and V. Kren: Biocatal. Biotransform. Vol. 20 (2002), p.73.

Google Scholar

[7] M. Kobayashi, T. Nagasawa and H. Yamada: Trends Biotechnol. Vol. 10 (1992), p.402.

Google Scholar

[8] C. D. Mathew, T. Nagasawa, M. Kobayashi and H. Yamada: Appl. Environ. Microbiol. Vol. 54 (1988), p.1030.

Google Scholar

[9] R. Shapiro, R. Dicosimo and S. M. Hennessey: Org. Proc. Res. Dev. Vol. 5 (2001), p.593.

Google Scholar

[10] P. G. Duan, S. Li, Y. Yang, Z. Z. Wang and L. Y. Dai: Chem. Eng. Technol. Vol. 32 (2009), p.771.

Google Scholar

[11] T. Matsuda, K. Yamada, Y. Shibata, H. Miura and K. Sugiyama: J. Chem. Soc. Faraday Trans. I. Vol. 83 (1987), p.3107.

Google Scholar

[12] Y. Asano, T. Yasuda and Y. Tani: Agric. Biol. Chem. Vol. 46 (1982), p.1183.

Google Scholar