Comparison of HPLC and Spectrophotometry Methods on Quantitative Determination of Formaldehyde in Water Based Coatings

Abstract:

To explore and compare the reliability of the quantitative determination of formaldehyde content of water based coatings, the high performance liquid chromatography (HPLC) after derivatization to 2.4-dinitrophenylhydazone and UV-VIS spectrophotometry were studied, respectively. HPLC was performed on a C₁₈ column with acetonitrile/water (65/35, V/V) as mobile phase at a flow rate of 1.0 mL/min, and analyses was detected using Photodode Array Detector (DAD) at the wavelength of 360 nm. And formaldehyde was determined with spectrophotometry at 435nm. The result showed the standard curve of formaldehyde by the HPLC method was linear in the range of 0.1-5.0 mg/L (R²=0.9998). The detection limit was 0.96mg/kg, recoveries were ranging from 96.2% to 101.3%. The linear range of formaldehyde by the spectrophotometric method was 0.1～5.0 mg/L (R²=0.9995), and the detection limit was 5.93mg/kg, recoveries were ranging from 98.5% to 105.3%. In conclusion, both the two methods can be used for quantitative analysis of formaldehyde in water based coatings, and the major advantages of HPLC are quick, simple and sensitive.

• Periodical: Advanced Materials Research (Volumes 816-817)
• Pages: 433-438
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.816-817.433
• Online since: September 2013
• Authors: Hai Hong Sun, Xiao Mei Mo, Ye Miao Qian
• Keywords: Formaldehyde, HPLC, Spectrophotometry, Water Based Coatings

References

[1] O. Bunkoed, F. Davis, P. Kanatharana, and P. Thavarungkul: Sol–gel based sensor for selective formaldehyde determination, Anal. Chim. Acta 659(2010) 251–257.

DOI: 10.1016/j.aca.2009.11.034

[2] X.F. Wang, B. Ding and M. Sun: Nanofibrous polyethyleneimine membranes as sensitive coatings for quartz crystal microbalance-based formaldehyde sensors, Sens. Actuators B 144 (2010) 11–17.

DOI: 10.1016/j.snb.2009.08.023

[3] C.H. Chou, J.L. Chang and J.M. Zen: Effective analysis of gaseous formaldehyde based on a platinum-deposited screen-printed edge band ultramicroelectrode coated with Nafion as solid polymer electrolyte, Sens. Actuators B 147 (2010) 669–675.

DOI: 10.1016/j.snb.2010.03.090

[4] Baez, A., et al., 2003. Carbonyl levels in indoor and outdoor air in Mexico City and Xalapa, Mexico. The Science of the Total Environment 302, 211–226.

DOI: 10.1016/s0048-9697(02)00344-3

[5] S. Barguil , Le Moullec, Y., and Person, A.: Chemical characterisation of indoor air quality in Parisian homes. Aerobiologia 6(1990), 28–31.

DOI: 10.1007/bf02539038

[6] De Bortoli, M.: Concentrations of selected organic pollutants in indoor and outdor air in Northern Italy. Environment International 12(1986), 343–350.

DOI: 10.1016/0160-4120(86)90048-6

[7] J. Jurvelin, M. Vartiainen, and M. Jantunen: Personal exposure levels and microenvironmental concentrations of formaldehyde and acetaldehyde in the Helsinki Metropolitan Area, Finland. Journal of Air Waste Management Associa-tion 51(2001), 17–24.

DOI: 10.1080/10473289.2001.10464251

[8] C. Marchand, B. Bulliot and S. Le Calve: Aldehyde measurements in indoor environment in Strasbourg (France). Atmospheric Environment 40(2006), 1336–1345.

DOI: 10.1016/j.atmosenv.2005.10.027

[9] P. Hu, N. Han, X. Zhang and M.S. Yao: Fabrication of ZnO nanorod-assembled multishelled hollow spheres and enhanced performance in gas sensor, J. Mater. Chem. 21 (2011) 14277–14284.

DOI: 10.1039/c1jm11919b

[10] Y.J. Lu, M. Meyyappan, J. Li: A carbon-nanotube-based sensor array for formaldehyde detection, Nanotechnology 22 (2011) 055502.

DOI: 10.1088/0957-4484/22/5/055502

[11] Z.J. Wang, J. Liu and F.J. Wang: Size-controlled synthesis of ZnSnO(3) cubic crystallites at low temperatures and their HCHO-sensing properties, J. Phys. Chem. C 114 (2010) 13577–13582.

DOI: 10.1021/jp104733e

[12] Pal, R. and Kim, K. H.: Experimental choices for the determination of carbonyl compounds in air. Journal of Separation Science, 30 (2007), 2708–2718.

DOI: 10.1002/jssc.200700206

[13] K.J. Dennis, T. Shibamoto, Gas chromatographic analysis of reactive carbonyl compounds formed from lipids upon UV-irradiation, Lipids 25 (1990) 460–464.

DOI: 10.1007/bf02538089

[14] Chen, J. W., Li, H. M. and Ding, F. L.: Determination of formaldehyde in soak of marine products with HPLC method. Preventive Medicine Tribune, 15(2009), 157–158.

[15] Pal, R. and Kim, K. H.: Influences of sampling volume and sample concentration on the analysis of atmospheric carbonyls by 2, 4-dinitrophenylhydrazine cartridge. Analytical Chimica Acta, 610(2008), 289–296.

DOI: 10.1016/j.aca.2008.01.034

[16] Saito,Y., Ueta, I., Ogawa, M. and Jinno, K.: Simultaneous derivatization preconcentration of volatile aldehydes with a miniaturized fiber-packed sample preparation device designed for gas chromatographic analysis. Analytical and Bioanalytical Chemistry, 386(2006).

DOI: 10.1007/s00216-006-0509-z

[17] Schulte, E.: Determination of higher carbonyl compounds in used frying fats by HPLC of DNPH derivatives. Analytical and Bioanalytical Chemistry, 372(2002), 644–648.

DOI: 10.1007/s00216-001-1228-0

[18] C. Marchand, B. Bulliot, and S. Le Calve: Aldehyde measurements in indoor environment in Strasbourg (France). Atmospheric Environment 40(2006), 1336–1345.

DOI: 10.1016/j.atmosenv.2005.10.027

[19] Wenhong Luo, Hui Li and Yuan Zhang: Determination of formaldehyde in blood plasma by high-performance liquid chromatography with fluorescence detection. Journal of Chromatography B, 753(2001): 253~257.

DOI: 10.1016/s0378-4347(00)00552-1

[20] Standard Method for Indoor Air Quality Measurement, GB/T 18883-2002, Chinese Standardization Administration of China, (2002).