Development of a Chemiluminescence ELISA for the Detection of Sudan I in Chilli Powder

Abstract:

A chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) was developed for the detection of Sudan I in food products. The CL-ELISA conditions, such as the concentration of antigen, antibody and goat anti-mouse IgG-HRP, were optimized. The optimized CL-ELISA allowed the Sudan I detection in a linear range of 0.625-10 ng mL^-1, the IC₅₀ was 3.3ng mL^-1 and the limit of detection (LOD) was 0.31 ng mL^-1. The method showed good recoveries with spiked chilli powder. The recovery rate in a batch range from 73-109.6%, and the recovery rate between the batch range from 78-109.24%. The proposed method proved to be efficient for the detection of Sudan I in food samples.

• Periodical: Advanced Materials Research (Volume 830)
• Pages: 310-313
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.830.310
• Online since: October 2013
• Authors: Yan Fan, Li Xin Zhu, Ren Rong Liu*, Long Xu, Wei Meng
• Keywords: Chemiluminescence, ELISA, Sudan i

References

[1] S.C. Buddhadasa, V. Mishra, A.K. Matta and N. Santhakumar: submitted to Food Australia (2007).

[2] F. Capitán, L.F. Capitán-Vallvey, et al: submitted to Analytica Chimica Acta (1996).

[3] Erdal Ertaş, Hayrettin Özer , Cesarettin Alasalvar: submitted to Food Chemistry (2007).

[4] Orawon Chailapakul, Wanida Wonsawat, et al: submitted to Food Chemistry (2008).

[5] Marie Stiborová, Václav Martínek, Helena Rýdlová, et al: submitted to Cancer Research (2002).

[6] Francesco Puoci, Carmelo Garreffa, Francesca Iemma, Rita Muzzalupo: submitted to Food Chemistry (2004).

[7] F. Tateo, M. Bononi: submitted to Journal of Agricultural and Food Chemistry (2004).

[8] M. Mazzetti, R. Fascioli, et al: submitted to Food Additive & Contaminants (2004).

[9] Vanessa Cornet, Yasmine Govaert, Goedele Moens, et al: submitted to Journal of Agricultural and Food Chemistry (2006).

[10] M.R.V.S. Murty, N. Sridhara Chary, S. Prabhakar: submitted to Food Chemistry (2009).