The Effect and Mechanism of Apoptosis on Hela Cells Induced by Bufotalin

Xu Chao; Xiao Jun Zhou; Chang Hu Dong; Gang Zheng

doi:10.4028/www.scientific.net/AMR.834-836.568

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 834-836The Effect and Mechanism of Apoptosis on Hela...

The Effect and Mechanism of Apoptosis on Hela Cells Induced by Bufotalin

Abstract:

Cytotoxic activity of Bufotalin was determined by MTT assay at various concentrations ranging from 0.002 to 0.008 μmol/ml on Hela cells. The Apoptosis and its mechanism induced by Bufotalin was also studied. The results showed Bufotalin displayed the marked inhibition effect to Hela cells and the IC₅₀value is 0.0045 μmol/ml for 24 hour. The procaspase-3,-8 and-9 decreased and their active cleaved forms increased treated with Bufotalin at concentrations from 0.002 to 0.006 μmol/ml. These data suggest that the pro-apoptotic effect of Bufotalin is mediated through activation of caspases and mitochondria in Hela cells.

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Periodical:

Advanced Materials Research (Volumes 834-836)

Pages:

568-572

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.834-836.568

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Online since:

October 2013

Authors:

Xu Chao, Xiao Jun Zhou, Chang Hu Dong, Gang Zheng*

Keywords:

Apoptosis, Bufotalin, Cytotoxicity, Hela

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