Bioremediation of Biofuel-Soil Contamination by Using Pseudomonas putida

Abstract:

Abstract. The commercialization of biodiesel/diesel blends on the market can cause environmental damages due to spills. This study aims to investigate the effectiveness of Pseudomonas putida as oil-biodegradable agent in soil contaminated with biodiesel/diesel blend (B20). The effectiveness on bioremediation have been conducted by examined several physico-chemical tests on biodiesel/diesel-contaminated soil before and after seeding of P. putida. The spillage stimulation of B20 was conducted at laboratory scale for 24 days of incubation time. The results show that the bioremediation treatment able to remove up to 82%, 77%, 16%, and 10% of nitrogen, phosphate, sulfate and total organic carbon, respectively. The pH of soil sample was changed from pH 7.45 (Day 0) to 7.25 (Day 24) after the treatment. Meanwhile, the moisture content in the sample has increased from 44.11% (Day 0) to 50.35% (Day 24). All of these results show the good indication of quality improvement of polluted soil after treated with P. putida. It is apparent from the acquired results that the application of P. putida is suitable as effective microorganism and potentially exploits as useful oil-soil biodegradable agent in polluted soil.

• Periodical: Advanced Materials Research (Volume 845)
• Pages: 138-145
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.845.138
• Online since: December 2013
• Authors: N.M. Sunar, Quin Emparan, Ahmad Tarmizi Abdul Karim, S.F.M. Noor, M. Maslan, F. Mustafa, N. Khaled
• Keywords: Biofuel, Bioremediation, Physico-Chemical Removal, Pseudomonas putida

References

[1] A.P. Mariano, R.C. Tomasella, D.L.M. Oliveira, J.C., De Angelis, Biodegradability of diesel and biodiesel blends. African Journal of Biotechnology, 7(9) (2008) pp.1323-1328.

[2] A., Demirbas. Biodiesel: a realistic fuel alternative for Diesel engines. London: Springer, (2008).

[3] Department of efficiency and renewable energy, U.S., Fact Sheet of biodiesel blends. Accessed on October 7, 2012, from http: /www. biodiesel. org/docs/ffs-basics/clean-cities-fact-sheet-biodiesel-blends-april 2008. pdf?sfvrsn=4.

DOI: 10.2172/1009258

[4] A. Demirbas, Progress and recent trends in biodiesel fuels. Energy conversion and management, 50 (2009) pp.14-34.

DOI: 10.1016/j.enconman.2008.09.001

[5] R.R. Eve, Remediation of petroleum contaminated soils: biological, physical and chemical process. USA: Lewis Publishers, (1998).

[6] G. Knothe, C.A. Sharp, T.W. Ryan, Exhaust emissions of biodiesel, petrodiesel, neat methyl esters, and alkanes in a new technology engine. Energy Fuels, 20(2006) pp.403-408.

DOI: 10.1021/ef0502711

[7] S.M., Mudge, G., Pereira Stimulating the biodegradation of crude oil with biodiesel preliminary results. Spill Sci Technol Bull, 5(1999) p.353–355.

DOI: 10.1016/s1353-2561(99)00075-4

[8] S.F. Cheng, Y.M. Chou, C.L. A.N.Y., Ma, Y., Basiron, Palm Biodiesel: Gearing toward Malaysian biodiesel standard. Malaysian palm oil board. Accessed on October 7, 2012, from: http: /www. americanpalmoil. com/pdf/biodiesel/gearing%20towards%20msian%20standards.

[9] H. Rubin, N. Narkis, J. Carberry, Soil and aquifer pollution-non-aqueous phase liquid-contamination and reclamation. 1st ed. Berlin: Springer. p.109, (1998).

DOI: 10.1007/978-3-662-03674-7

[10] T. Watanabe, T. Hirayama, Genotoxicity of soil. J. Health Sci., 47(2001) pp.433-438.

[11] Environmental Protection Agency (EPA). Assessment and remediation of contaminated sediments (ARCS) program, final summary report, EPA-905-S-94-001, EPA, Chicago.

DOI: 10.1017/s0376892900039060

[12] J. Walworth, P. Andrew, J. Rayner, S. Ferguson, P., Harvey, Fine turning soil nitrogen to maximize petroleum bioremediation. Assessment and remediation contaminated sites in Arctic and cold climates (ARCSACC), (2005) pp.251-257.

[13] D.G. Linz, D. V Nakles, Environmentally acceptable endpoint in soil. American academy of environmental engineers, Annapolis, MD. (1997) pp.121-147.

[14] R. Kucerova, Application of Pseudomonas putida and Rhodococcus spp. by biodegradation of PAHs, PCBs and NEL soil samples from the hazardous waste dump in Pozdatky . Mining-Geological-Petroleum Engineering Bulletin, 18, (2006) pp.97-101.

[15] A.K. Haritash, C.P. Kaushik, Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): a review, J. Hazard. Matter, 169, (2009). p.1–15.

DOI: 10.1016/j.jhazmat.2009.03.137

[16] K.N. Timmis,. Pseudomonas putida: a cosmopolitan opportunist par excellent. Environ Microbiol., 4, (2002) pp.779-781.

DOI: 10.1046/j.1462-2920.2002.00365.x

[17] I.P. Castro, J. Becker, K. Dohnt, Dos V.M. Santos, C. Wittmann,. Industrial biotechnology of Pseudomonas putida and related species. App. Microbiol Biotechnol., 93, (2012) pp.2279-2290.

DOI: 10.1007/s00253-012-3928-0

[18] V.A.P. Martins Dos Santos, S. H. Moore, M. Stratz, K.N. Timmis, Insights into the genomic basis of niche specificity of Pseudomonas putida 2440. Environ Microbiol., 6, (2004) pp.1264-1286.

DOI: 10.1111/j.1462-2920.2004.00734.x

[19] Z. A Malik, S. Ahmed, Degradation of petroleum hydrocarbons by oil field isolated bacterial consortia, African Journal of Biotechnology, 11(3) (2012), pp.650-658.

[20] S. B. Hamed, A. Maaroufi, A. Ghram, B.A.G. Zouhaier, M. Labat,. African Journal of Biotechnology, 12(14), (2013) pp.1636-1643.

[21] L.T. Taylor, D.M. Jones, Bioremediation of coal tar PAH in soil using biodiesel, Chemosphere 44, (2001) p.1131–1136.

DOI: 10.1016/s0045-6535(00)00344-1

[22] P.E. Jackson, Ion chromatography in environmental analysis. Encylopedia of analytical chemistry, (2000) pp.2779-2801.

[23] A.P. Mariano, R.C. Tomasella, L.M. De Oliveira, J.C.D.D.F. De Angelis, Biodegradability of diesel and biodiesel blends. African Journal of Biotechnology, 7(9), (2008) pp.1323-1328.

[24] A. Demirbas, Progress and recent trends in biodiesel fuels. Energy conversion and management, 50, (2009) pp.14-34.

DOI: 10.1016/j.enconman.2008.09.001

[25] A.C. Pinto, L.L.N. Guarieiro, M.J.C. Rezende, N.M. Ribeiro, E.A. Torres, W.A. Lopes, P.A.P. Pereira, J.B. Andrade, Biodiesel: An overview. J. Braz. Chem. Soc., 16(6B), (2005) pp.1313-1330.

DOI: 10.1590/s0103-50532005000800003

[26] Fangruima, M.A. Hanna, Biodiesel Production: A review. Bioresource Technology, 1, (1999) pp.1-15.

[27] The United States Department of Agriculture Natural Resources Conservation Service (USDA) Soil survey staff, keys to soil taxonomy, 8th edition, Washington, DC: Natural Resources, (1998).

DOI: 10.17138/tgft(2)188-196