Study on Properties of PG Separated and Purified from Actinidia arguta

Abstract:

Polygalacturonase (PG) is key enzyme for softening consenescence mechanism of fruits. In this work, Properties of PG separated and purified from wild Actinidia Arguta were studied. The result shows that the optimal reaction temperature is 40°C with a high enzyme activity around 30-50°C. PG keeps a thermo stability at the temperature ranging from 30-40°C, which decreases with increasing of temperature and PG will lose all its activity under 60-70°C for 1h. The optimal reaction pH value for this enzyme is 5.5. As far as the stability in different pH values is concerned, PG is relatively stable for preservation at pH4.5-6.5.The michaelis constant for PG is 27.31. When the concentration for EDTA is higher than 2mmol/L, it is inhibitive for PG . The metal ions of K⁺, Ca²⁺, Fe²⁺, Fe³⁺, Mg²⁺ can activate the PG enzyme to some extent, the strength of activation:Mg²⁺Fe³⁺K⁺Fe²⁺Ca²⁺;The metal ions of Cu²⁺Zn²⁺Mn²⁺Ba²⁺Al³⁺ can inhibit PG enzyme to some extent, the strength of inhibition:Al³⁺Ba²⁺Mn²⁺ Cu²⁺Zn²⁺.

• Periodical: Advanced Materials Research (Volumes 850-851)
• Pages: 1197-1201
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.850-851.1197
• Online since: December 2013
• Authors: Shu Qian Li*, Chang Jiang Liu, Guang Xin, Bo Zhang
• Keywords: Actinidia arguta, PG, Property

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