Expression of Costimulatory Molecules B7-H4 in PBC Patients

Chen Zhao; Yang Zhao; Yong Zhe Li; Chao Jun Hu; Ding Hua Liu

doi:10.4028/www.scientific.net/AMR.850-851.1216

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 850-851Expression of Costimulatory Molecules B7-H4 in PBC...

Expression of Costimulatory Molecules B7-H4 in PBC Patients

Abstract:

Objective：To study the relationship between the levels of B7-H4 and PBC. Methods：Real-time quantitative PCR were used to detect the levels of B7-H4 mRNA on PBMCs. ELISA were used to detect the serum levels of IL-2. FACs were used to detect the CD4+and CD8+ T-lymphocytes frequencies. Results：Levels of B7-H4 mRNA and B7-H4 frequencies were significantly lower than non-PBC hepatic cirrhosis group and healthy controls (P<0.01). After PHA treated for 72h the frequencies of CD4+, CD8+, and CD4+CD8+ T-lymphocytes were all decreased (P<0.05). The same with serum levels of IL-2 (P<0.05). And the frequencies of CD4+ and CD4+CD8+ T-lymphocytes and serum levels of IL-2 were significantly higher in the PBC group than that of non-PBC hepatic cirrhosis group and healthy controls (P<0. 01). Conclusion：Levels of B7-H4 decreased in PBC and could play an important role in immunoregulation of PBC.

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Periodical:

Advanced Materials Research (Volumes 850-851)

Pages:

1216-1219

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.850-851.1216

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Online since:

December 2013

Authors:

Chen Zhao*, Yang Zhao, Yong Zhe Li, Chao Jun Hu, Ding Hua Liu

Keywords:

B7-H4, Costimulation Molecular, peripheral blood mononuclear cells, Primary Biliary Cirrhosis

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