Study on Compositional Change of Berchemia lineata from Different Harvest Times

Abstract:

To study content of quercetin in Zhuang folk medicine Berchemia lineata in the different harvest times and to explore the laws of dynamic changes of quercetin content. Methods: determine 10 batches of Berchemia lineata in the HPLC-DAD method. Chromatographic conditions: Agilent XDB C₁₈(150 mm×4.6 mm, 5 μm), the column temperature of 25 °C; mobile phase of methanol (A) - acetonitrile (B) -0.05% phosphoric acid solution (C), gradient elution; volume flow of 1 mL/min, detection wavelength of 254 nm. Result: sample injection volume of quercetin in the range of 0.69 μg ~ 4.83 μg has a good linear relationship with peak area, the average recovery is 99.8%, RSD: 1.27% (n = 6). From the overall analysis, content of quercetin in Berchemia lineata harvested in January is higher than that in other months. Conclusion: January should be the best month for harvest of Berchemia lineata.

• Periodical: Advanced Materials Research (Volumes 850-851)
• Pages: 1267-1270
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.850-851.1267
• Online since: December 2013
• Authors: Hong Li Teng*, Li Cheng Guo, Shi Lin Chen
• Keywords: Berchemia lineata, Fingerprint, Gradient Elution, HPLC-DAD

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