Comparing the Properties of Relatively High Concentration LPA by Capillary Electrophoresis

Abstract:

Two relatively high concentration 4% and 6% the replaceable Linear polyacrylamide (LPA) gel was used as the sieving medium. An extensive series of experiments has been performed using DNA fragments ranging in size from 80 to 587 base pairs; the properties such as the mobilities, the width at half height, and the resolution were compared. The electric field was changed from 100V/cm to 375V/cm. From the experiment results, it is shown: increasing the gel concentration from 4%LPA to 6%LPA can not improve the resolution to separate the DNA sample (the length of fragments600bp). This work complements our systematic study and will help to further optimize and improve high performance DNA separation in microfluidic applications.

• Periodical: Advanced Materials Research (Volumes 850-851)
• Pages: 1283-1286
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.850-851.1283
• Online since: December 2013
• Authors: Jie Wang*, Wei Wan
• Keywords: Concentration, Linear Polyacrylamide (LPA), Resolution

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