Determination of Ferulic Acid in “AI-FU-NUAN-GONG” Pills by High Performance Capillary Electrophoresis

Abstract:

High performance capillary zone electrophoresis (HPCE) was used in this text to determine the content of ferulic acid in a ready-made Chinese herbal medicine called AI-FUNUAN-GONG (in pills). Electrophoretic separation conditions: Uncoated fused silica capillary column (75um×49/58cm), injection height 7.5cm, 30mmolL^-1 borax solution, separation voltage 20kV, injection time 10s, detection wavelength 315 nm, experimental temperature 20°C.Linearity was kept in the concentration ranging from 1~62.5 mg·L^-1 of ferulic acid with correlation coefficient of 0.970. The average recovery was 97.541% and RSD value was 6.57%. Sample content of ferulic acid was 0.0715mg/g. This method has the advantages of strong specificity, simpleness, speediness, accurate results and good repeatability in separation and it is useful for determination of ferulic Acid in AI-FU-NUAN-GONG Pills.

• Periodical: Advanced Materials Research (Volumes 850-851)
• Pages: 57-61
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.850-851.57
• Online since: December 2013
• Authors: Hai Xing Liu*, Qing Liu, Xia Chen, Yan Fu Huan, Lin Tong Wang, Kai Qi Ye, Guang Zeng
• Keywords: AI-FU-NUAN-GONG Pills, Determination, Ferulic Acid, High Performance Capillary Zone Electrophoresis

References

[1] Pang Yanling, Wang huaiyou. Chinese Journal of Analysis Laboratory, 2008, 01, 60-62.

[2] Liao Lv, Zhou Mingda, Xiao Jin, Chou Jixiong. Science and Technology of Food Industry, 2007, 02, 217-219.

[3] Chen Yanjun. Shandong Chemical Industry, 2013, 01, 48-50.

[4] Liu Qian, Ding Qiuling, Bao Li, Zhang Fenlong. Chin J Mod Drug Appl, 2011, 5(22), 69-70.

[5] Zhao Qinglan, Yang Jing, Bai Bing, Li Mingjing, Journal of Henan University, 2009, 03, 256-258.

[6] Hu Yanfeng. Chinese Journal of Analysis Laboratory. 2008, S2, 399-400.