Effect of Exogenous Calcium on Root Growth and Endogenous Hormone Contents in Pineapple Seedlings

Abstract:

As an essential element, calcium plays a key role in plant development. The present study aimed at assessing the effects of exogenous calcium on root activity, plant growth and endogenous hormone contents in pineapple seedlings. Major experimental methods included hydroponic culture with six concentrations (0, 5, 10, 20, 40, 80 mg/L) of CaCl₂ and the use of high performance liquid chromatography (HPLC) for its endogenous hormone contents. After cultivating for 48 days, the seedlings were sampled and the reduction of TTC (2,3,5-triphenyitetrazolium chloride) method was determined to assess the root activity, and the determination of endogenous hormone contents was carried out by HPLC. The results showed that the shoot fresh weight, the root activity, root length and root weight increased significantly in response to the 20 mg/L Ca²⁺ treatment, and all these parameters seemed to be suppressed at higher Ca²⁺ concentrations. The contents of endogenous hormone ZT, GA₃ and IAA were evidently higher at 40 mg/L Ca²⁺, with ZT, GA₃ and IAA reach values as high as 2.31, 31.48 and 16.57 μg/g, respectively, while the highest concentration of ABA ( 0.026 μg/g) appeared at 5 mg /L Ca²⁺ concentration.

• Periodical: Advanced Materials Research (Volumes 864-867)
• Pages: 106-110
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.864-867.106
• Online since: December 2013
• Authors: Ying Dui He, Rui Mei Li, Chang Bin Wei, Guang Ming Sun
• Keywords: Ananas comosus, Endogenous Hormone, Root Activity, Root Growth, Root Physiology

References

[1] Datnoff, L.E., Elmer, W.H., Huber, D.M.: APS Press, St. Paul, MN. (2007).

[2] Medvedev S. S: Russian Journal of Plant Physiology, 52(2) (2005) 249-270.

[3] Kent L.M., Läuchli A.: Plant. cell and Environment. 8 (1995) 155-159.

[4] El-Hamdaoui A., Redondo-Nieto M., Torralba B., Rivilla R. Bonilla I. Bolaños L.: Plant and Soil. 251 (2003) 93-103.

DOI: 10.1023/a:1022980330718

[5] Maeda Y., Nakazawa R.: Biologia Plantarum. 52(1) (2008) 153-156.

[6] Zieslin N., Abolitz M., : Ann. Bot. 92 (2003) 487-511.

[7] Guo Y., sun X. Z., Song X.L., Wang Q.C., Li Y.J., Chen S.Y.,: Plant nutrition and fertilizer science. 12(3) (2006) 363-368.

[8] Zhao P., Lin K.H., Zhen Y.: Plant nutrition and fertilizer science. l1(3)(2005) 379-384.

[9] Xu J., Chen S.W.: Acta horticulturae sinica. 16(4) (2002) 275-278.

[10] Steponkus P.L., Lanphear F.O.: Plant Physiol. 42 (1967) 1423-1426.

[11] Ceng Q.Q., Chen H.B., Lu C.H., Li J.G.: Journal of fruit science. 23(1) (2006)145-148.

[12] Wu Y.S., Hu Y.B., Xu G.H.: Plant Growth Regul. 57 (2009) 271-280.

[13] Hoveland C.S.: The Georgia Cattleman. (1999) 19.

[14] Feagley S.E., Fenn L.B.: Texas Agricultural Extension Service. (1998).

[15] Schwartzkopf C.: USGA Green section Record. (1972).

[16] Loneragan J.F., Snowball K., Simmons W.J.: Aust. J. Agric. Res. 19 (1968) 845-857.

[17] Lund Z.F.,: Soil Sci. Soc. Am. Proc. 34 (1970) 456-459.

[18] Marschner H., Richter C.: Plant Soil. 40 (1974) 193-210.

[19] Kopittke P.M., Menzies N.W.: Plant Soil. 269 (2005) 119-129.

[20] Pintro J.C., Taylor G.J.,: J. Plant. Nutr. 28 (2005) 551-565.

[21] Teiceira A.L., Aneliz de Bastos Andrade. Ferrarese-Filho O., Maria de Lourdes Lucio Ferrarese: Plant Growth Regulation. 49 (2006) 69-76.

DOI: 10.1007/s10725-006-0013-7

[22] White P.J., Broadley M.R.: Ann. Bot. 92(2003) 487-511.

[23] Davies P.J.: In: Davies P.J. (Eds), Plant Hormones, Physiology, Biochemistry and Molecular Biology, Kluwer Academic Publishers Dordrecht, Boston, London, (1995).

DOI: 10.1006/anbo.1996.0152

[24] Weyers J.D.B., Paterson N.W. : New Phytol. 152 (2001) 375-407.

[25] Bhatt K., Sarma A., Thaker V.: Indian Journal of Experimental Biology. 46 (2008) 673-676.

[26] Kovaleva L.V., Zakharova E.V., Minkina Yu.V., Timofeeva G.V., Andreev I.M.: Russian Journal of Plant Physiology. 52(4) (2005) 584-590.

[27] Ivanov I.I.: Russian Journal of Plant Physiology, 56(2) (2009) 219-223.