Scale-Up Production of Bacillus Thuringiensis by Box-Type Solid-State Fermentation Equipment

Li Na Qiu; Wei Wei Zhang; Ai Jun Gong; Ru Lei Yao

doi:10.4028/www.scientific.net/AMR.864-867.299

Paper Titles

Effects of DOM on the Migration of Cr (VI) in Soils
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The Changes of Heavy Metals Solubility with Time under Different Chelants in Contaminated Soil
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Composition and Antioxidant Capacity of Flour and Hull Extracts from Different Tartary Buckwheat Cultivars
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Effect of La (III) on some Physiological Index and Ultrastructure of Honeysuckle (Lonicera japonica Thunb.)
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Scale-Up Production of Bacillus Thuringiensis by Box-Type Solid-State Fermentation Equipment
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The Experiment Study of the Pollution of Phthalate Compounds and Heavy Metals to Rice in the Film
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Utilizing UV Mutagenesis to Breeding Strains with High Ability of Oil Shale Degradation
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A Study on the Distribution and Correlation of Small Phytoplankton and Nutrients (N, P) in Spring in the Xiangshan Harbor
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Different Patterns of Ionic Liquids-Regenerated Cellulose Carriers for Papain Immobilization
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 864-867Scale-Up Production of Bacillus Thuringiensis by...

Scale-Up Production of Bacillus Thuringiensis by Box-Type Solid-State Fermentation Equipment

Abstract:

The Bacillus thuringiensis (Bt) which is considered as the most effective bio-pesticide is widely used in the world. There are mainly two modes for the production of this microbial pesticides: submerged fermentation and solid-state fermentation. In this paper, The Scale-up of Kg-class Bt production was studied on wheat bran based media in the Box-type solid-state fermentation equipment made by our lab. The fermentation conditions were optimized and the result show that, Bt production yielded a significant increase in spore count and toxin content at the following conditions: actiactivation of culture for 8h, initial moisture content of solid substrate 55%, pH value 7.5, keeping the thick of inoculated media about 4cm and fermenting for 45 h, Maintaining the temperature within a range of 25-30°C, The bioassay efficacy of the product could be above 13000IU·mg^-1, The production of Bt kilograms class by solid-state fermentation was successfully represented as well as providing a strong technical support to the large-scale industrial production of Bt bio-pesticide.