Different Patterns of Ionic Liquids-Regenerated Cellulose Carriers for Papain Immobilization

Ya Mei Yuan; Qiu Jin Li; Song Kun Yao; Ji Xian Gong; Jian Fei Zhang

doi:10.4028/www.scientific.net/AMR.864-867.319

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 864-867Different Patterns of Ionic Liquids-Regenerated...

Different Patterns of Ionic Liquids-Regenerated Cellulose Carriers for Papain Immobilization

Abstract:

Different patterns (beads, membranes and powders) of cellulose regenerated from room temperature ionic liquid 1-butyl-3-methyl imidazolium chloride ([Bmim]Cl) were prepared to immobilized papain molecules. It is found that regenerated cellulose (RC) membranes was the best pattern for papain loading and immobilization. Removing the supernatant of the solution containing free enzyme as well as RC carriers before immobilization without the addition of ethanol as the precipitant were benefit for increasing the activity of the immobilized papain. Papain could be immobilized successfully on the surface of RC carriers through SEM analysis.

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Periodical:

Advanced Materials Research (Volumes 864-867)

Pages:

319-323

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.864-867.319

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Online since:

December 2013

Authors:

Ya Mei Yuan, Qiu Jin Li, Song Kun Yao, Ji Xian Gong, Jian Fei Zhang

Keywords:

Carrier Pattern, Enzyme Activity, Ionic Liquid (IL), Papain Immobilization, Regenerated Cellulose

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