Optimum Conditions for Lipase Immobilization on Halloysitum Rubrum

Tao Deng; Jun Wei Xu; Li Huang; Tao Li; Xu Ya Yu

doi:10.4028/www.scientific.net/AMR.864-867.465

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 864-867Optimum Conditions for Lipase Immobilization on...

Optimum Conditions for Lipase Immobilization on Halloysitum Rubrum

Abstract:

In this study, we use natural halloysitum rubrum as novel support materials to immobilize Candida rugosa lipase. The response surface methodology with a four-factor three-level Box-Behnken experimental design was used to evaluate the effects of immobilization parameters, such as pH (4.0 to 6.0), immobilization temperature (25 °C to 35 °C), enzyme/support ratio (0.1 to 0.3, w/w), and immobilization time (1 h to 2 h), on the activity of immobilized lipase. The optimum pH, temperature, enzyme/support ratio, and time for immobilized lipase activity (376.09 U/g) were 5.17, 29.65 °C, 0.3 (w/w), and 1.63 h, respectively. After 15 repeated uses, the immobilized lipase still retained 80% of its initial activity, which indicates good reusability.

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Periodical:

Advanced Materials Research (Volumes 864-867)

Pages:

465-471

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.864-867.465

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Online since:

December 2013

Authors:

Tao Deng, Jun Wei Xu, Li Huang, Tao Li, Xu Ya Yu*

Keywords:

Halloysitum Rubrum, Immobilized Lipase, Response Surface Methodology (RSM)

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* - Corresponding Author

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