The Influence of Glucose on Growth and Lipid Production under Heterotrophic and Mixotrophic Conditions for Monoraphidium sp. FXY-10

Abstract:

The impact of glucose on the growth under heterotrophic and mixotrophic conditions for Monoraphidium sp. FXY-10 as well as its lipid content was investigated through the Andrew equation. The results demonstrated that the subsaturation constant of Monoraphidium sp. FXY-10 under heterotrophic condition to glucose was smaller than that of Monoraphidium sp. FXY-10 under mixotrophic condition, indicating that Monoraphidium sp. FXY-10 is more sensitive to glucose under heterotrophic condition. Furthermore, the biomass productivity, lipid productivity and lipid content of Monoraphidium sp. FXY-10 all reached the peak at 10 g L-1 of glucose concentration under both heterotrophic and mixotrophic conditions. Compared with other Chlorella sp., the Monoraphidium sp. FXY-10 represented higher lipid productivity (131.79 mg L^-1 d^-1).

• Periodical: Advanced Materials Research (Volumes 864-867)
• Pages: 49-55
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.864-867.49
• Online since: December 2013
• Authors: Yong Teng Zhao, Peng Zhao, Tao Li, Lin Wang, Xu Ya Yu*
• Keywords: Glucose, Heterotrophic, Lipid Productivity, Mixotrophic, Monoraphidium Sp. FXY-10

References

[1] S. Shafiee, E. Topal, When will fossil fuel reserves be diminished? , Energ Policy. 37 (2009)181-189.

DOI: 10.1016/j.enpol.2008.08.016

[2] C. Bigogno, I.K. Goldberg, S. Boussiba, A. Vonshak, Z. Cohen, Lipid and fatty acid composition of the green oleaginous algae Parietochloris incise, the richest plant source of arachidonic acid, Phytochemistry. 60(2002)497-503.

DOI: 10.1016/s0031-9422(02)00100-0

[3] G. Antolin, F.V. Tinaut, Y. Briceno, Optimisation of biodiesel production by slmtlower oil transesterification, Bioresource Technology. 83(2002)111-114.

DOI: 10.1016/s0960-8524(01)00200-0

[4] H.A. Tamarys, W. Wei, H. Bo, Oil accumulation via heterotrophic/mixotrophic Chlorella protothecoides, Appl Biochem Biotech. 162(2010)1978-(1995).

DOI: 10.1007/s12010-010-8974-4

[5] Y. Liang, N. Sarkany, Y. Cui, Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions, Biotechno Lett. 31(2009) 1043-1049.

DOI: 10.1007/s10529-009-9975-7

[6] M.X. Wan, R.M. Wang, J.L. Xia, J.N. Rosenberg, Z.Y. Nie, N. Kobayashi, G.A. Oyler, M.J. Betenbaugh, Physiological evaluation of a new Chlorella sorokiniana isolate for its biomass production and lipid accumulation in photoautotrophic and heterotrophic cultures, Biotechnol Bioeng. 109(2012).

DOI: 10.1002/bit.24477

[7] X.Y. Yu, P. Zhao, C.A. He, J.J. Li, X.H. Tang, J.P. Zhou, Z.X. Huang, Isolation of a novel strain of Monoraphidium sp. and characterization of its potential application as biodiesel feedstock, Bioresource Technology. 121(2012)256-262.

DOI: 10.1016/j.biortech.2012.07.002

[8] E.G. Bligh, W.J. Dyer, Extraction of Lipids in Solution by the Method of Bligh ＆ Dyer, Can. J. Biochem. Physiol. 37 (1959) 911-917.

DOI: 10.1139/o59-099

[9] H.Y. Wang, H.R. Xiong, Z.L. Hui, Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids, Bioresource Technology. 104(2012) 215-220.

DOI: 10.1016/j.biortech.2011.11.020

[10] X W Zhang, X M Shi, F Chen, A kinetic model for lutein production by the green microalga Chlorella protothecoides in heterotrophic culture,J. Ind Micobiol Biot. 23(1999)503-507.

DOI: 10.1038/sj.jim.2900760

[11] F.M. M Morel, Kinetics of nutrient uptake and growth in phytoplankton,J. Phycol. 23 (1987)137-150.

[12] M.J. Griffiths, S.T. L. Harrison, Lipid productivity as a key characteristic for choosing algal species for biodiesel production , J. Appl Phycol. 215(2009)493-507.

DOI: 10.1007/s10811-008-9392-7

[13] Q. Hu, M. Sommerfeld, E. Jarvis, M. Ghirardi, M. Posewitz, M. Seibert, A. Darzins, Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances, Plant J. 54(2008) 621-639.

DOI: 10.1111/j.1365-313x.2008.03492.x

[14] Y. Chisti, Biodiesel from microalgae beats bioethanol, Trends Biotechno, 26(2008)126-131.

DOI: 10.1016/j.tibtech.2007.12.002