Application of a Bioinformatics Method on Detecting of Acinetobacter baumannii Carrying Metallo-β-Lactamases Isolated from Inpatients

Abstract:

The aim of the study was to investigate the prevalence and charateristics of Acinetobacter baumannii producing MBLs isolated from the several wards of the affiliated hospital in Jilin regions. The bioinformatics technology was used to design primers, sequence and blast, in conjunction with antibiotic susceptibility test and MBL screening method. The total of 119 were isolated from three wards and 88 strains (73.9 %) produced MBLs, the number of 31 strains (26.1%) didnt produce MBLs. All MBLs isolates showed same antimicrobial susceptibility patterns. Among Acinetobacter baumannii producing MBLs, 78 strains (88.9%) were positive for bla_OXA-23 gene, 10 strains (11.1%) contained bla_VIM-2 gene. Plasmid profiles and pulsed-field gel electrophoresis were used to study the relatedness of isolates. All MBLs isolates were closely related by PFGE and plasmid analysis. This study confirmed the potential risk of spreading of Acinetobacter baumannii producing MBLs with inter-and intrahospital clonal transfer of patients.

• Periodical: Advanced Materials Research (Volumes 881-883)
• Pages: 335-338
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.881-883.335
• Online since: January 2014
• Authors: Lin Lin Ma, Ali Huang, Wei Chu, Xiao Han Li, Ming Cheng Li
• Keywords: Acinetobacter Baumannii, Bioinformatics, Metallo-β-Lactamases, Nosocomial Infection

References

[1] Montefour K, Frieden J, Hurst S, et al: submitted to Crit Care Nurse (2008).

[2] Bayuga S, Zeana C, Sahni J, et al: submitted to Heart Lung (2002).

[3] Alsultan AA, Hamouda A, Evans BA, et al: submitted to J Chemother (2009).

[4] Hasan B, Perveen K, Olsen B, et al: submitted to J Med Microbiol (2013).

[5] Sarı AN, Biçmen M, Gülay Z: submitted to Jpn J Infect Dis (2013).

[6] Gales AC, Jones RN, Sader HS submitted to: Clin Microbiol Infect (2006).

[7] Gordon NC, Wareham DW submitted to Int J Antimicrob Agents (2010).

[8] Yu YS, Qu TT, Zhou JY, et al: submitted to J Clin Microbiol (2006).

[9] Clinical and Laboratory Standards Institute, CLSI document M100-S15, Wayne, PA (2005).

[10] Ben Othman A., Zribi M., Masmoudi A., et al: submitted to Braz. J. Microbiol (2011).

[11] Turton JF, Kaufmann ME, Gill MJ, et al: submitted to J Clin Microbiol (2006).

[12] Tenover FC, Arbeit RD, Goering RV, et al: submitted to J Clin Microbiol (1995).

[13] Dong F, Xu X and Song WQ: submitted to Chin J Microbiol Immunol (Chin) (2006).

[14] Queenan A., Bush K: submitted to Clin. Microbiol. Rev (2007).

[15] Qu TT, Zhang JL, Wang J: submitted to J Clin Microbiol (2009).

[16] Gaynes R, Edwards JR: submitted to Clin Infect Dis (2005).

[17] Takagi E., Lincopan N., Cassettari V.: submitted to Braz. J. Microbiol (2009).

[18] Jang TN, Lee SH, Huang CH, et al: submitted to J Hosp Infect (2009).

[19] Safari M, Saidijam M, Bahador A, et al: submitted to J Res Health Sci (2013).

[20] Gulay Z, Atay T and Amyes SG: submitted to J Chemother agent (2001).

[21] Fouad M, Attia AS, Tawakkol WM, et al: submitted to Int J Infect Dis (2013).