Spectrophotometric Determination of 6-Mercaptopurine in Pharmaceutical Sample Using Fe(III)-Potassium Ferricyanide System

Abstract:

In the present work,we developed a simple,fast,sensitive and inexpensive method to determine 6-mercaptopurine (6-MP) in pharmaceutical sample using Fe (III)-potassium ferricyanide system by spectrophotometry.The results show that in acid medium, Fe (Ш) can be reduced to Fe (II) by hydrosulfuryl (-SH) in 6-Mercaptopurin molecule, and then Fe (II) reacts with potassium ferricyanide to form a soluble Prussian blue (KFe^III[Fe^II(CN)₆]),the content of 6-MP was determinated indirectly through determinating the absorbance of the soluble Prussian blue.The various effect factors on the determination of 6-MP by spectrophotometry using potassium Fe (III)-ferricyanide system were investigated in detail.The maximum absorption wavelength of chromogenic system was 755 nm, good linear relationship was obtained between the absorbance and the concentration of 6-MP in the range of 0.4120~2.884 μ g·/mL,the equation of the linear regression was A=0.0411+0.1462C (μ g·/mL),with a linear correlation coefficient was 0.9991.This proposed method had been successfully applied to determinate of 6-MP in real pharmaceutical,and the results agreed well with those obtained by pharmacopoeial method.

• Periodical: Advanced Materials Research (Volumes 881-883)
• Pages: 479-483
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.881-883.479
• Online since: January 2014
• Authors: Chang Qing Tu, Xin Rong Wen
• Keywords: 6-Mercaptopurine, Ferric Chloride, Potassium Ferricyanide, Prussian Blue, Spectrophotometry

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