Isolation of Endophytic Fungi from Ephedra intermedia and Research Antibacterial Activity of Secondary Metabolite Produced by the Fungi

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Abstract:

20 strains of endophytic fungi were isolated from roots and stems of Ephedra intermedia. After fermentation these strains, fermentation broth and mycelium were extracted by ethyl acetate, n-butanol and methyl alcohol, producing 60 extracts. Then the antibacterial activity of these extracts was researched, the result found that 33 extracted samples could evidently inhibit the growth of six different bacteria, accounted for 55%. The MIC of ethyl acetate extract of J109 was 0.15625mg/mL to five test bacterial expect Streptococcus uberi.

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Advanced Materials Research (Volumes 881-883)

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488-492

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January 2014

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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[1] Petrini O, Fisher PJ, Petrini LE. Fungal endophytes of bracken (Pteridium aquilinum), with some reflections on their use in biological control. Sydowia 1992; 44: 282–293.

Google Scholar

[2] U'Ren JM, Lutzoni F, Miadlikowska J, Arnold E. Community analysis reveals close affinities between endophytic and endolichenic fungi in mosses and lichens. Microb Ecol2010; 60: 340–353.

DOI: 10.1007/s00248-010-9698-2

Google Scholar

[3] Soca-Chafre G, Rivera-Ordun˜a FN, Hidalgo-Lara ME, Hernan-dez Rodriguez C, Marsch R, Flores-Cotera LB. Molecular phylogeny and paclitaxel screening of fungal endophytes from Taxus globosa. Fungal Biol 2011; 115: 143–156.

DOI: 10.1016/j.funbio.2010.11.004

Google Scholar

[4] Pinruan U, Rungjindamai N, ChoeyklinR, Lumyong S, Hyde KD, Jones EBG. Occurrence and diversity of basidiomycetous endophytes from the oil palm, Elaeis guineensis in Thailand. Fungal Divers 2010; 41: 1–71.

DOI: 10.1007/s13225-010-0029-1

Google Scholar

[5] Vaz ABM, Mota RC, Bomfim MRQ, Zani CL, Rosa CA, Rosa LH. Antimicrobial activity of endophytic fungi associated with Orchidaceae in Brazil. Can J Microbiol 2009; 55: 1381–1391.

DOI: 10.1139/w09-101

Google Scholar

[6] Bills GF, Polishook JD. Microfungi from Carpinus caroliniana. Can J Bot 1991; 69: 1477-1482.

DOI: 10.1139/b91-191

Google Scholar

[7] Strobel GA, Daisy B, Castillo U, Harper J. Natural Product from endophytic microorganisms. J Nat Prod 2004; 67: 257-268.

DOI: 10.1021/np030397v

Google Scholar