Efficient Desizing of Fabrio Using Partially Purified Thermophillic Amylase Produced by T. sacchari by the Utilization of Agro-Industrial Wastes

Abstract:

Thermoactinomyces sacchari was isolated from hot water springs.Then by utilizing the solid state fermentation techniques, amylase was obtained, which was partially purified. The partially pu rified enzyme was then used to remove the starch size from cloth. Effective enzymatic desizing requires the strict control of pH, temperature, water hardness, electrolyte addition and choice of surfactant. It was observed that maximum removal 7.5 g was achieved by soaking the cloth in 100 ml of 0.1M HCl for 2 hours. A maximum of 98.4desizing was obtained after 90 minutes at 55oC. A maximum desizing of 98.9 was obtained after 60 minutes of incubation at 55oC.A maximum desizing of100% was obtained at pH 6.0 at 55oC for 60 minutes. At lower and higher values desizing decreased. When cotton pieces were soaked for 60 minutes at pH 6.0, maximum desizing (100%) was obtained at 60oC.

• Periodical: Advanced Materials Research (Volume 924)
• Pages: 349-353
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.924.349
• Online since: April 2014
• Authors: Muhammad Ayub Khan*, Muhammad Zahid Qureshi, Lorna Dawson, Mujadad Rehman, Azam Hayat, Farhana Maqbool, Farah Andleeb, Kashif Haleem
• Keywords: Agro-Industrial Waste, Starch Desizing , T. sacchari , Thermophillic Amylase

References

[1] Crabb, W. D., Shetty, J. K. (1999). Commodity scale production of sugars from starches. Curr. Opin. Microbiol., 2: 252–256.

DOI: 10.1016/s1369-5274(99)80044-7

[2] Crabb, W.D. and Mitchinson, C. 1997. Enzymes involved in the processing of starch to sugars . BTECH. 15: 349-352.

DOI: 10.1016/s0167-7799(97)01082-2

[3] Haki, G. D. and Rakshit, S. K. (2003).

[4] Ikram-ul-Haq, Ashraf H, Iqbal J and Qadeer MA (2003) Production of alpha amylase by Bacillus licheniformis using an economical medium. Bioresource Technology, 87: 57–61.

DOI: 10.1016/s0960-8524(02)00198-0

[5] Review. Bioresource Technology, 89: 17-34.

[6] Masahiro,S., Takashi,E. andRitsuka, O. 1997. Improvement of detergency oflaundry bleach compositions and bleaching method without color darkening. Microbia., 5(25): 1010-1020.

[7] Appleyard, H. (1953). Enzyme action in desizing. Dyer., 109: 685-687.

[8] Onzales . M. A 1997. Laundary detergent bar composition with bleach system stabilized by enzymes . Av. Appl., 9: 110-115.

[9] Pandey, A., Nigam, P., Soccol, C. R., Soccol, V. T., Singh, D. and Mohan, R. (2000). Advances in microbial amylases. Biotechnol. Appl. Biochem., 31: 135–152.

DOI: 10.1042/ba19990073

[10] . Allan, S., Torbenvedel, B. and Henrik, B. F. (1997). Recombinant alpha amylase mutants and their use in textile desizing, starch liquefaction and washing. PTC. Int. Appl., 12(5): 205-210.

[11] Allan., S., Henrik., B. F and torbendevel, B 1996. A method of desizing alpha mutants , with predetermined properties alpha amylase variants and detergents containing the variants Process B iochenm., 31(5): 110-210.

[12] Goyal,S. G and Khandeparkar,V. G 1978 Amylase from Bacillus subtilis159 for desizing . Indian journal. Text. Res.

[13] Iqbal. j. Yousaf,M. Chaudry.M. Y and Hamid, M. 1997. Desizing enzyme fromBacillus subtilis234. Pak.J. Sci., 49(1&2): 1-3.

[14] Julicher,W. 1952EnzymicDesizing of fabrics . Melliand. Textilber., 33: 511-516.

[15] Abate, C.M., Castro, G.R., Sineriz, F., Callieri, D. (1999). Production of amylolytic enzymes by Bacillus emyloliquefaciens in pure culture and in co-culture with Zymomonasmobilis. Biotechnology, Letters., 21 (3): 249-252.

[16] Adinarayana K, Kugen, P. andSuren S. (2005). Amylase production in solid state fermentation by the thermophilic fungus Thermomyceslanuginosus. J. Biosci. Bioeng. 100 2): 168-171.

[17] Bernier, R., Kopp, M., Trakas, B., and Stutzenberger, F. (2008). Production of extracellular enzymes by Thermomonosporacurvata during growth on protein-extracted lucernefibres. Journal of Applied Microbiology, 65(5): 411 – 418.

DOI: 10.1111/j.1365-2672.1988.tb01910.x

[18] Cheng-Hsiung Chang and Shang-Shyng Yang (2009). Thermo-tolerant hosphatesolubilizing Microbes for multi-functional biofertilizer preparation. Bioresource Technology(100) 1648–1658.

DOI: 10.1016/j.biortech.2008.09.009

[19] Haq, I., Ashraf, H., Qadeer, M. A., Javed, I. (2005). Pearl millet, a source of alpha amylase production by Bacillus licheniformis. Biores. Technol., 96: 1201–1204.

DOI: 10.1016/j.biortech.2004.09.012

[20] Van Der MarrelMJEC. Van Der VeenB, UitedhaangJCM, LeehiusH, Dijkhuizen L 2002. Properties and applications of staech converting enzymes of Alpha amylase family. J. Biotechnol, 94: 137-155.

DOI: 10.1016/s0168-1656(01)00407-2

[21] Peixoto, S. C., Jorge, J. A., Terenzi, H. F., DeLourdes, M. and Polizeli, T. M. (2003). Rhizopusmicrosporus var. rhizopodiformis: a thermotolerant fungus with potential for production of thermostable amylases. Int. Microbiol. 6: 269-273.

DOI: 10.1007/s10123-003-0140-1

[22] Poonam, N. and Dalel, S. (1995). Enzyme and microbial systems involved in starch processing. Enzyme Microb. Technol. 17: 770-778.

DOI: 10.1016/0141-0229(94)00003-a

[23] Kakumanu, P., Wilhelm, H, T,., Ryohei,O., Ceser,B. A and Soroh,R. 1997. Dingy fabric cleanup with amylase in detergent compositions P.C.T. Int Appl. 21(5): 333-340.