Immobilization of SA-α-2,6-Gal Receptors Related to Influenza on Magnetic Nanoparticles Coated with Chitosan

Karla M. Gregorio-Jauregui; Susana A. Carrizales-Alvarez; Jorge E. Rivera-Salinas; Hened Saade; Jose L. Martinez; Raul G. Lopez; Anna Ilyina

doi:10.4028/www.scientific.net/AMR.976.19

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 976Immobilization of SA-α-2,6-Gal Receptors Related...

Immobilization of SA-α-2,6-Gal Receptors Related to Influenza on Magnetic Nanoparticles Coated with Chitosan

Abstract:

Influenza infection is carried out due to the virus hemagglutinin recognizes host cell surface with terminal sialic acid (SA) linked receptors SA-α-2,6-Gal for human strain. Sambucus nigra lectin has structural similarity to the viral hemagglutinin. Magnetic nanoparticles (MNP) coated with chitosan can be used as support for the study of these receptors. The goal of this study was to extract the SA-α-2,6-Gal receptors of porcine trachea for their immobilization on magnetic nanoparticles coated with chitosan. The extraction was carried out based on affinity of Sambucus nigra lectin to SA-α-2,6-Gal receptors. It was possible to immobilize up to 76% of SA-α-2,6-Gal receptors, with a molecular weight at 88.4 kDa for more representative protein. The presence of the receptors was confirmed trough FTIR analysis. Magnetic functionalized nanoparticles showed superparamagnetic properties and an average diameter around 10 nm. These results may be used to evaluate the interaction between functionalized nanoparticles and specific lectin or hemagglutinin of influenza virus as model of study virus-receptor.

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Periodical:

Advanced Materials Research (Volume 976)

Pages:

19-24

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.976.19

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Online since:

June 2014

Authors:

Karla M. Gregorio-Jauregui, Susana A. Carrizales-Alvarez, Jorge E. Rivera-Salinas, Hened Saade, Jose L. Martinez, Raul G. Lopez, Anna Ilyina*

Keywords:

Magnetic Nanoparticles, Sambucus nigra Lectin, SA-α-2,6-Gal Receptors

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