Application of Ultrasonic Treatment on Collecting Recombinant Protein from Yeast

Jia Kun Dai; Yan Li; Hui Hui Zhuang; Xi Chen; Qi Ma; Sheng Yun Xu; Tao Qin; Ya Hui Wei

doi:10.4028/www.scientific.net/AMR.998-999.191

Paper Titles

Analysis of Factors and Characteristics in Patients with Hand-Foot-Mouth Disease
p.173

Aneuploidy Alnus formosana Selection and ISSR Analysis
p.177

Anti-Tumor Effects of a Newly Cloned Serine Protease from the Marine Annelid, Arenicola cristata
p.183

Application of Carbon Nanoparticles as Fluorescent Quenchors in Biochemistry
p.187

Application of Ultrasonic Treatment on Collecting Recombinant Protein from Yeast
p.191

Biodistribution and Toxicity Study of Titanium Dioxide Nanoparticles of Different Sizes after Intravenous Injection in Mice
p.196

Cardioprotective Effects of Aqueous Extract of Salvia miltiorrhiza on Heart Failure is Related to SDF-1/CXCR4 Axis and Bcl-2 Family Expressions
p.200

Cloning and Activity Analysis of a New Protease from Arenicola cristata
p.206

Cloning and Expression of a Novel Protease with Fibrinolytic Activity from Arenicola cristata
p.210

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 998-999Application of Ultrasonic Treatment on Collecting...

Application of Ultrasonic Treatment on Collecting Recombinant Protein from Yeast

Abstract:

An appropriate ultrasonication of collecting foreign recombinant protein (human-adiponectin) from yeast was established. Regarded the bioactivity of foreign recombinant protein tested by Western Blotting analysis as parameter, a L9(3³) orthogonal array design based on a series of single-factor experiments was employed to optimize conditions for ultrasonic disruption. The results showed that the ultrasonic power of 450 W, duration time on 25 min and operation interval (work time: intermittent time) of 10:10 (s/s) were optimal process with the highest protein bioactivity, and meanwhile, the cell breaking rate was (67.8±2.1) %.

You might also be interested in these eBooks

Advances in Applied Sciences, Engineering and Technology II

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 998-999)

Pages:

191-195

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.998-999.191

Citation:

Cite this paper

Online since:

July 2014

Authors:

Jia Kun Dai, Yan Li, Hui Hui Zhuang, Xi Chen, Qi Ma, Sheng Yun Xu, Tao Qin, Ya Hui Wei*

Keywords:

Human-Adiponectin Protein, Ultrasonication, Yeast

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

* - Corresponding Author

References

[1] M. Ángeles Pozo-Bayón, Inmaculada Andújar-Ortiz, M. Victoria Moreno-Arribas. Scientific evidences beyond the application of inactive dry yeast preparations in winemaking, J. Food Research International. 42(2009)754-761.

DOI: 10.1016/j.foodres.2009.03.004

Google Scholar

[2] Renata G. K. Leuschner, Jan Bew, Gérard Bertin. Validation of an official control method for enumeration of authorised probiotic yeast in animal feed, J. System and Appled microbiology. 26 (2003)147-153.

DOI: 10.1078/072320203322337443

Google Scholar

[3] Qun Wu, Liangqiang Chen, Yan Xu. Yeast community associated with the solid state fermentation of traditional Chinese Maotai-flavor liquor, J. International Journal of Food Microbiology. 166(2013)323-330.

DOI: 10.1016/j.ijfoodmicro.2013.07.003

Google Scholar

[4] FINJI AKADA. Genetically modified industrial yeast ready for application, J. Journal of Bioscience and Bioengineering. 94(2002) 536-544.

DOI: 10.1016/s1389-1723(02)80192-x

Google Scholar

[5] Vivek D. Farkade, Susan T.L. Harrison, Aniruddha B. Pandit. Improved cavitational cell disruption following pH pretreatment for the extraction of β-galactosidase from Kluveromyces lactis, J. Biochemical Engineering Journal. 31(2006)25-30.

DOI: 10.1016/j.bej.2006.05.015

Google Scholar

[6] Kwang Suk Kim, Jung Eun Chang, Hyun Shik Yun. Estimation of soluble β-glucan content of yeast cell wall by the sensitivity to Glucanex® 200G treatment, J. Enzyme and Microbial Technology. 35(2004)672-677.

DOI: 10.1016/j.enzmictec.2004.08.020

Google Scholar

[7] H. Anand, B. Balasundaram, A.B. Pandit, et al. The effect of chemical pretreatment combined with mechanical disruption on the extent of disruption and release of intracellular protein from E. coli, J. Biochemical Engineering Journal. 35(2007).

DOI: 10.1016/j.bej.2007.01.011

Google Scholar

[8] Gustavo Ciudad, Olga Rubilar, Laura Azócar, et al. Performance of an enzymatic extract in Botrycoccus braunii cell wall disruption, J. Journal of Bioscience and Bioengineering. 36 (2013) 1-6.

DOI: 10.1016/j.jbiosc.2013.06.012

Google Scholar

[9] L.M. Monks, A. Rigo, M.A. Mazutti, et al. Use ofchemical, enzymatic and ultrasound-assisted methods for cell disruption to obtain carotenoids, J. Biocatalysis and Agricultural Biotechnology. 2(2013)165-169.

DOI: 10.1016/j.bcab.2013.03.004

Google Scholar

[10] Chin Woi Ho, Tsuey Kee Chew, Tau Chuan Ling, et al. Efficient mechanical cell disruption of Escherichia coli by an ultrasonicator and recovery of intracellular hepatitis B core antigen, J. Process Biochemistry. 41(2006)1829-1834.

DOI: 10.1016/j.procbio.2006.03.043

Google Scholar

[11] A. Lateef, J.K. Oloke, S.G. Prapulla. The effect of ultrasonication on the release of fructosyl transferase from Aureobasidium pullulans CFR 77, J. Enzyme and Microbial Technology. 40(2007) 1067-1070.

DOI: 10.1016/j.enzmictec.2006.08.008

Google Scholar

[12] Dan Liu, Xin-An Zeng, Da-Wen Sun. Disruption and protein release by ultrasonication of yeast cells, J. Innovative Food Science and Emerging Technologies. 18(2013)132-137.

DOI: 10.1016/j.ifset.2013.02.006

Google Scholar

[13] Liu Y, UNO H, TAKATSUKI H, et al. Interrelation between HeLa- S3 cell transfection and hemolysis in red blood cell suspension using pulsed ultrasound of various duty cycles, J. European Biophysics Journal. 34(2005) 163-169.

DOI: 10.1007/s00249-004-0439-7

Google Scholar