Cloning and Expression of a Novel Protease with Fibrinolytic Activity from Arenicola cristata

Chun Ling Zhao; Wen Jing Yu; Ji Yu Ju

doi:10.4028/www.scientific.net/AMR.998-999.210

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 998-999Cloning and Expression of a Novel Protease with...

Cloning and Expression of a Novel Protease with Fibrinolytic Activity from Arenicola cristata

Abstract:

cDNA of a novel protease, designated as AFEI, was cloned from digestive tract of Arenicola cristata by RACE. The cDNA of AFEIcomprised 897bp and an open reading frame that encoded polypeptides of 264 amino acid residues. AFEIshowed similarity to serine protease family and contained the conserved catalytic amino acid residues. The gene encoding the active form of AFEIwas expressed in E.coli and the purified recombinant protein could dissolve an artificial fibrin plate with plasminogen, which indicated the recombinant protein might be a plasminogen activator for thrombosis therapy.

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Periodical:

Advanced Materials Research (Volumes 998-999)

Pages:

210-213

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.998-999.210

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Online since:

July 2014

Authors:

Chun Ling Zhao, Wen Jing Yu, Ji Yu Ju*

Keywords:

Arenicola cristata, Fibrinolytic Enzyme, Gene Sequence, Race

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* - Corresponding Author

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