Protective Effects of Quercetin Derivatives against Hydrogen Peroxide-Induced Oxidative Damage in PC-12 Cells

Abstract:

In order to search for new structural modification strategies on quercetin, we have designed and synthesized a series of quercetin derivatives. Several novel quercetin derivatives displayed potent protect effect on H₂O₂-induced injury in PC12 cells. In the recent study, we investigated the effect of 3,3',4',5,7-pentahydroxyflavone 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one (HPS5) on the apoptosis of PC12 cells in vitro. We used neuronal PC12 cell line to study the regulation role of HPS5 in H₂O₂ induced cell apoptosis. MTT assay was used for detection the proliferation of PC12 cells upon H₂O₂ treatment. PC12 cell nuclear morphological change was detected with Hoechst 33258 staining. The antioxidative capacity was judged by SOD determinations. The protein levels of Bax and Bcl-2 were deteanined by Western blotting. Our results demonstrated that Quercetin derivatives HPS5 can prevent and protect against H₂O₂-induced injury in PC12 cells. Together, our studies provided new insights activities of quercetin, and may contribute to pharmacological study of quercetin in future research.

• Periodical: Advanced Materials Research (Volumes 998-999)
• Pages: 291-295
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.998-999.291
• Online since: July 2014
• Authors: Zhen Yu Shi*, Yong Qiang Li
• Keywords: Apoptosis, Oxidative Damage, PC12 Cells, Quercetin

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