Stem Cell-Like Characteristics of Corneal Epithelial Cells

Fan Wang; Bo Ren; Yi Ning Yan

doi:10.4028/www.scientific.net/AMR.998-999.312

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 998-999Stem Cell-Like Characteristics of Corneal...

Stem Cell-Like Characteristics of Corneal Epithelial Cells

Abstract:

Purpose: The adult corneal epithelium is maintained by a population of limbal stem cells (LSCs), transmembrane protein prominin, regarded as stem cell marker was investigated on mouse corneal tissue, to study weather contains CD133-expressing cells and their distribution. Methods: Enucleated mouse eyes were embedded in OCT and cryosections were performed for mmunohistochemical studies using the avidin-biotin-peroxidase complex (ABC) procedure. Meanwhile, dissected mouse corneas were analyzed by westernblot. Results: In the adult mouse, 13A4 immunoreactivity was detected at the apical side of superficial corneal epithelium, including the limbus region, but not by stroma and endothelium. 115 KDa protein was approved in corneal tissue by Westernblot. Conclusions: The stem cell activity does not occur along the limbus but presumably presented by small portion of corneal epithelial cells which may hold a similar properties of stem cells.

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Periodical:

Advanced Materials Research (Volumes 998-999)

Pages:

312-315

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.998-999.312

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Online since:

July 2014

Authors:

Fan Wang, Bo Ren*, Yi Ning Yan

Keywords:

Epithelium, Limbus, Prominin-1/CD133, Stem Cell

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