Study on Compatibility of Self-Assembly Peptide RADA16-I, PLGA and PS Scafolds and BMSCs

Abstract:

Objective: To observe the ability of self-assembly peptide RADA16-І hydrogel, poly (lactic-co-glycolic acid) (PLGA) and polystyrene (PS) scaffolds in supporting the adherent growth of rat bone marrow-derived mesenchymal stem cells (BMSCs). Methods: The rat BMSCs were isolated and purified by density gradient centrifugation and adherent culture. The 3^rd passage of BMSCs were used to induced into adipocytes and osteoblasts by inductive medium in vitro, and labeled by infection of adenovirus carried with green fluorescent protein. BMSCs were inoculated on RADA16-І hydrogel, PLGA and PS scaffolds to form cell-scaffold complex. Adhesion rates of BMSCs on different scaffolds were investigated, and cell proliferation was tested by MTT method. Results: Cultured BMSCs presented high uniformity and had multilineage differentiation potential. Adhesion rates of BMSCs on RADA16-І hydrogel, PLGA and PS scaffolds were 92.51%, 78.36% and 59.79% respectively. BMSCs cultured on RADA16-І hydrogel showed higher ability of proliferation than those cultured on PLGA and PS scaffolds after incubation for 7 days (P < 0.05). Conclusion: RADA16-І hydrogel is more suitable than PLGA and PS for attachment and growth of BMSCs.

• Periodical: Advanced Materials Research (Volumes 998-999)
• Pages: 325-329
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.998-999.325
• Online since: July 2014
• Authors: Lan Wang, Yan Sheng Liao, Chao Xian Yang*
• Keywords: Bone Marrow-Derived Mesenchymal Stem Cells, Poly(lactic-Co-Glycolic Acid), Polystyrene, Self-Assembling Peptide RADA16-I

References

[1] X. Liu, X. Wang, X. Wang, H. Ren, J. He, L. Qiao and F.Z. Cui: Acta Biomater, Vol. 9 (2013) No. 6, p.6798.

[2] F. Xu, Y. Wang, X. Jiang, H. Tan, H. Li and K.J. Wang: Kaohsiung J Med Sci, Vol. 28 (2012) No. 1, p.10.

[3] M. Sato, M. Ishihara, N. Kaneshiro, G. Mitani, T. Nagai, T. Kutsuna, T. Asazuma, M. Kikuchi and J. Mochida: J Biomed Mater Res B Appl Biomater, Vol. 83 (2007), No. 1, p.181.

DOI: 10.1002/jbm.b.30782

[4] J. Shi, X. Zhang, X. Zeng, J. Zhu, Y. Pi, C. Zhou and Y. Ao: Orthopedics, Vol. 35 (2012), No. 5, p. e665.

[5] M. Wang, H. Pei, L. Zhang, L. Guan, R. Zhang, Y. Jia, B. Li, W. Yue, Y. Wang and X. Pei: Tissue Eng Part C Methods, Vol. 16 (2010), No. 5, p.1041.

[6] J. Wang, Q. Yang, C. Mao and S. Zhang: J Biomed Mater Res A, Vol. 100 (2012), No. 11, p.2929.

[7] S. Li, A.J. Hayes, B. Caterson and C.E. Hughes: Histochem Cell Biol, Vol. 139 (2013), No. 1, p.59.

[8] R. Hashimoto, Y. Katoh, K. Nakamura, S. Itoh, T. Iesaki, H. Daida, Y. Nakazato and T. Okada: Biochem Biophys Res Commun, Vol. 423 (2012), No. 4, p.672.

[9] B. Cai, J. Li, J. Wang, X. Luo, J. Ai, Y. Liu, N. Wang, H. Liang, M. Zhang, N. Chen, G. Wang, S. Xing, X. Zhou, B. Yang, X. Wang and Y. Lu: Stem Cells, Vol. 30 (2012), No. 8, p.1746.

DOI: 10.1002/stem.1154

[10] X. Tian, S. Wang, Z. Zhang and D. Lv: PLoS One, Vol. 7 (2012), No. 8, p. e42804.

[11] C. Yang, L. Zhou, X. Gao, B. Chen, J. Tu, H. Sun, X. Liu, J. He and Q. Yuan: Neurosurgery, Vol. 68 (2011), No. 3, p.691.

[12] Z. Feng, C. Li, S. Jiao, B. Hu and L. Zhao: Hepatogastroenterology, Vol. 58 (2011), No. 112, p. (2081).

[13] R.J. 'Keefe and J. Mao: Tissue Eng Part B Rev, Vol. 17 (2011), No. 6, p.389.

[14] X. Miao, D.M. Tan, J. Li, Y. Xiao and R. Crawford: Acta Biomater, Vol. 4 (2008), No. 3, p.6.