Synthesis and Detection Experiments of a Biomolecule Detection Probe Based on Fluorescence Changes

Abstract:

The sensitivity of fluorescence probes built upon the resonance energy transfer is not high enough at present. We built a fluorescence probe with high sensitivity (SA-488-sub-nanogold) by means of the fluorochrome Alexa488 (SA-488) labeled by streptavidin, nanogold, and biotin-subpeptide. When the fluorescence molecule SA-488 binds with the nanogold by biotin-subpeptide, the fluorescence intensity will be suppressed because of resonance energy transfer. If there are molecules under test, the energy transfer will be blocked, by which we can get the molecule content from the fluorescence intensity. Using this probe, we acquired a lower detection limit and a higher sensitivity for biotin detection.

• Periodical: Advanced Materials Research (Volumes 998-999)
• Pages: 336-339
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.998-999.336
• Online since: July 2014
• Authors: Jun Wang, Da Hai Ren*
• Keywords: Fluorescent Probe, Nanogold, Resonance Energy Transfer, MEMS

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