Study on Phenol-Degrading Characteristics of Hybrid Strains and Immobilized Cells

Abstract:

This study aims to enhance the bacteria’s ability to degrade phenol by exploring the phenol-degrading capability of hybrid strains and immobilized cells. Two phenol-degradation strains named as A1 and A2 were isolated respectively.The StrainA1 was identified as Burkholderia, while strain A2 was found to be Bacillus cereuswere. In addition, conditions including inoculum strains , pH value, temperature and dissolved oxygen were tested. . It was shown that: (1)Hybrid strains ( mixed A1 with A2) was better than single strain(A1 or A2) in degrading phenol because of the Synergistic effect. It can completely degraded phenol at the concentration of 300mg/ L, 500mg / L, 700mg / L within 12h, 24h, 48h respectively. (2) The optimum environmental conditions of phenol degradation for hybrid strains culture is 35°C, pH is 7.0, the inoculum strains is 10%. (3) It is beneficial to the degradation of phenol, when the speed of the shaker was improved. (4) Phenol-degradation rate and the tolerance of high concentration of phenol were significantly improved when the A1 and A2 were mixed immoblized.

• Periodical: Advanced Materials Research (Volumes 998-999)
• Pages: 330-335
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.998-999.330
• Online since: July 2014
• Authors: Cai Hong Yu*, Wan Zhong Zhang, Chun Yan Li, Wen Juan Wang, Ying Huang
• Keywords: Biodegradation, Hybrid, Immobilized Cell, Phenol

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