Detection of Cancer Cells Using G-Rich DNA Based Target Binding-Switching Calorimetric Biosensor

Abstract:

This paper reports the G-rich ssDNA for the colorimetric detection of cancer cells. The ssDNA-1 sequence has explored for the potential application of “Turn-On” colorimetric sensor for selective and sensitive detection of cancer cells. While complementary G-rich DNA strand form G-quadruplex with hemin molecule, which is more effective to catalyze the peroxidase mimicking activity towards TMB chromogenic substrate. The ssDNA-1 exhibits good selectivity for cancer cells. The colorimetric intensity of TMB was enhanced upon interaction of leukemic lymphoblasts cancer cells. The effect of pH has turned the selective sensing performances of the biosensor for detecting cancer cells with a lower detection limit of 0.54 nM, 0.18 nM, and 0.2 nM respectively.

• Periodical: Advances in Science and Technology (Volume 117)
• Pages: 73-78
• DOI: https://doi.org/10.4028/p-3o604e
• Online since: August 2022
• Authors: Alisir Babakuliyev, Niladri Maiti, Annie Aglin Antony, Mohammad Javed Ansari, Santosh S. Chobe, Chandra Kumar Dixit*
• Keywords: Cancer Cells, Colorimetric Sensor, Peroxidase Mimics, Ssdna, TMB

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