Electrospun Biomaterials’ Applications and Processing

Abstract:

One of the largest fields of application of electrospun materials is the biomedical field, including development of scaffolds for tissue engineering, drug delivery and wound healing. Electrospinning appears as a promising technique in terms of scaffolds composition and architecture, which is the main aspect of this review paper, with a special attention to natural polymers including collagen, fibrinogen, silk fibroin, chitosan, chitin etc. Thanks to the adaptability of the electrospinning process, versatile hybrid, custom tailored structure scaffolds have been reported. The same is achieved due to the vast biomaterials’ processability as well as modifications of the basic electrospinning set-up and its combination with other techniques, simultaneously or by post-processing.

• Periodical: Journal of Biomimetics, Biomaterials and Biomedical Engineering (Volume 49)
• Pages: 91-100
• DOI: https://doi.org/10.4028/www.scientific.net/JBBBE.49.91
• Online since: February 2021
• Authors: Lucie Depeigne, Emilija Zdraveva*
• Keywords: Applications, Biomaterials, Biomedicine, Electrospinning, Tissue Engineering

References

[1] E. Zdraveva, J. Fang, B. Mijovic, T. Lin. Electrospun nanofibers, in: G. Bhat, editor. Structure and Properties of High-Performance Fibers, Elsevier, UK, 2017, pp.267-300.

DOI: 10.1016/b978-0-08-100550-7.00011-5

[2] D. Annis, A. Bornat, R. Edwards, A. Higham, B. Loveday, J. Wilson, An elastomeric vascular prosthesis, ASAIO Journal, 24 (1978) 209-214.

[3] A. Messina, L. De Bartolo. Polymeric Membranes for the Biofabrication of Tissues and Organs, in: G. Forgacs, W. Sun, (Eds.), Biofabrication, Elsevier, 2013, pp.81-94.

DOI: 10.1016/b978-1-4557-2852-7.00005-6

[4] N.H.A. Ngadiman, M. Noordin, A. Idris, D. Kurniawan, A review of evolution of electrospun tissue engineering scaffold: From two dimensions to three dimensions, Proc. Inst. Mech. Eng. H, 231 (2017) 597-616.

DOI: 10.1177/0954411917699021

[5] A. Sensini, L. Cristofolini, Biofabrication of electrospun scaffolds for the regeneration of tendons and ligaments, Materials, 11 (2018) (1963).

DOI: 10.3390/ma11101963

[6] L. Vogt, L. Liverani, J.A. Roether, A.R. Boccaccini, Electrospun zein fibers incorporating poly (glycerol sebacate) for soft tissue engineering, Nanomaterials, 8 (2018) 150.

DOI: 10.3390/nano8030150

[7] A.E. Erickson, D. Edmondson, F.-C. Chang, D. Wood, A. Gong, S.L. Levengood, et al., High-throughput and high-yield fabrication of uniaxially-aligned chitosan-based nanofibers by centrifugal electrospinning, Carbohydr. Polym., 134 (2015) 467-474.

DOI: 10.1016/j.carbpol.2015.07.097

[8] A. Magiera, J. Markowski, E. Menaszek, J. Pilch, S. Blazewicz, PLA-based hybrid and composite electrospun fibrous scaffolds as potential materials for tissue engineering, J. Nanomater., 2017 (2017) 1-11.

DOI: 10.1155/2017/9246802

[9] Z. Wu, B. Kong, R. Liu, W. Sun, S. Mi, Engineering of corneal tissue through an aligned PVA/collagen composite nanofibrous electrospun scaffold, Nanomaterials, 8 (2018) 124.

DOI: 10.3390/nano8020124

[10] D. Liang, B.S. Hsiao, B. Chu, Functional electrospun nanofibrous scaffolds for biomedical applications, Adv. Drug Deliv. Rev., 59 (2007) 1392-1412.

DOI: 10.1016/j.addr.2007.04.021

[11] Y. Luu, K. Kim, B. Hsiao, B. Chu, M. Hadjiargyrou, Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA–PEG block copolymers, J. Controlled Release, 89 (2003) 341-353.

DOI: 10.1016/s0168-3659(03)00097-x

[12] Y. Maghdouri-White, S. Petrova, N. Sori, S. Polk, H. Wriggers, R. Ogle, et al., Electrospun silk–collagen scaffolds and BMP-13 for ligament and tendon repair and regeneration, Biomed. Phys. Eng. Express., 4 (2018) 025013.

DOI: 10.1088/2057-1976/aa9c6f

[13] L. Meng, O. Arnoult, M. Smith, G.E. Wnek, Electrospinning of in situ crosslinked collagen nanofibers, J. Mater. Chem., 22 (2012) 19412-19417.

DOI: 10.1039/c2jm31618h

[14] A.J. Tabor, A. Robinson, B.I. Pinto, R.S. Kellar, Platelet rich plasma combined with an electrospun collagen scaffold: In-vivo and invitro wound healing effects, Clin. Res. Dermal Open Access, 3 (2016) 1-8.

DOI: 10.15226/2378-1726/3/2/00125

[15] S. Agarwal, J.H. Wendorff, A. Greiner, Use of electrospinning technique for biomedical applications, Polymer, 49 (2008) 5603-5621.

DOI: 10.1016/j.polymer.2008.09.014

[16] B. Kong, S. Mi, Electrospun scaffolds for corneal tissue engineering: A review, Materials, 9 (2016) 614.

[17] D.A. Parry, J.M. Squire, Fibrous proteins: Coiled-coils, collagen and elastomers, first ed., Gulf Professional Publishing, (2005).

DOI: 10.1016/s0065-3233(05)70001-2

[18] R.L. Reis, N.M. Neves, J.F. Mano, M.E. Gomes, A.P. Marques, H.S. Azevedo, Natural-based polymers for biomedical applications, Elsevier, (2008).

DOI: 10.1016/b978-1-84569-264-3.50034-2

[19] G.E. Wnek, M.E. Carr, D.G. Simpson, G.L. Bowlin, Electrospinning of nanofiber fibrinogen structures, Nano Lett., 3 (2003) 213-216.

DOI: 10.1021/nl025866c

[20] R.F. Doolittle, Fibrinogen and fibrin, Annu. Rev. Biochem, 53 (1984) 195-229.

DOI: 10.1146/annurev.bi.53.070184.001211

[21] J.W. Weisel. Fibrinogen and fibrin, in: D. Parry, A. D., J.M. Squire, (Eds.), Advances in protein chemistry, 70, Elsevier, 2005, pp.247-299.

[22] L.-D. Koh, Y. Cheng, C.-P. Teng, Y.-W. Khin, X.-J. Loh, S.-Y. Tee, et al., Structures, mechanical properties and applications of silk fibroin materials, Prog. Polym. Sci., 46 (2015) 86-110.

DOI: 10.1016/j.progpolymsci.2015.02.001

[23] A.J. Meinel, K.E. Kubow, E. Klotzsch, M. Garcia-Fuentes, M.L. Smith, V. Vogel, et al., Optimization strategies for electrospun silk fibroin tissue engineering scaffolds, Biomaterials, 30 (2009) 3058-3067.

DOI: 10.1016/j.biomaterials.2009.01.054

[24] P. Zhou, G. Li, Z. Shao, X. Pan, T. Yu, Structure of Bombyx mori silk fibroin based on the DFT chemical shift calculation, J. Phys. Chem. B, 105 (2001) 12469-12476.

DOI: 10.1021/jp0125395

[25] C. Viney, From natural silks to new polymer fibres, J. Text. I., 91 (2000) 2-23.

[26] P.K. Dutta, J. Dutta, V. Tripathi, Chitin and chitosan: Chemistry, properties and applications, J. Sci. Ind. Res., 63 (2004) 20-31.

[27] S. Şenel, S.J. McClure, Potential applications of chitosan in veterinary medicine, Adv. Drug Deliv. Rev., 56 (2004) 1467-1480.

DOI: 10.1016/j.addr.2004.02.007

[28] A. Hasan, A. Memic, N. Annabi, M. Hossain, A. Paul, M.R. Dokmeci, et al., Electrospun scaffolds for tissue engineering of vascular grafts, Acta Biomater., 10 (2014) 11-25.

DOI: 10.1016/j.actbio.2013.08.022

[29] D.A. Parry, A.S. Craig. Collagen fibrils during development and maturation and their contribution to the mechanical attributes of connective tissue, Collagen, CRC Press, 2018, pp.1-23.

[30] E.D. Boland, J.A. Matthews, K.J. Pawlowski, D.G. Simpson, G.E. Wnek, G.L. Bowlin, Electrospinning collagen and elastin: preliminary vascular tissue engineering, Front Biosci, 9 (2004) e32.

DOI: 10.2741/1313

[31] L. Liverani, N. Raffel, A. Fattahi, A. Preis, I. Hoffmann, A.R. Boccaccini, et al., Electrospun patterned porous scaffolds for the support of ovarian follicles growth: a feasibility study, Sci. Rep., 9 (2019) 1-14.

DOI: 10.1038/s41598-018-37640-1

[32] N.L.B.M. Yusof, A. Wee, L.Y. Lim, E. Khor, Flexible chitin films as potential wound‐dressing materials: Wound model studies, J. Biomed. Mater. Res. A, 66 (2003) 224-232.

DOI: 10.1002/jbm.a.10545

[33] K.E. Park, H.K. Kang, S.J. Lee, B.-M. Min, W.H. Park, Biomimetic nanofibrous scaffolds: preparation and characterization of PGA/chitin blend nanofibers, Biomacromolecules, 7 (2006) 635-643.

DOI: 10.1021/bm0509265

[34] I. Jun, H.-S. Han, J.R. Edwards, H. Jeon, Electrospun fibrous scaffolds for tissue engineering: Viewpoints on architecture and fabrication, Int. J. Mol. Sci., 19 (2018) 745.

DOI: 10.3390/ijms19030745

[35] R.J. Stoddard, A.L. Steger, A.K. Blakney, K.A. Woodrow, In pursuit of functional electrospun materials for clinical applications in humans, Ther. Deliv., 7 (2016) 387-409.

DOI: 10.4155/tde-2016-0017

[36] S. Gnavi, B.E. Fornasari, C. Tonda-Turo, R. Laurano, M. Zanetti, G. Ciardelli, et al., The effect of electrospun gelatin fibers alignment on schwann cell and axon behavior and organization in the perspective of artificial nerve design, Int. J. Mol. Sci., 16 (2015) 12925-12942.

DOI: 10.3390/ijms160612925

[37] E. Yu, J. Zhang, J. Thomson, L.-S. Turng, Fabrication and characterization of electrospun thermoplastic polyurethane/fibroin small-diameter vascular grafts for vascular tissue engineering, Int. Polym. Proc., 31 (2016) 638-646.

DOI: 10.3139/217.3247

[38] D. Li, T. Wu, N. He, J. Wang, W. Chen, L. He, et al., Three-dimensional polycaprolactone scaffold via needleless electrospinning promotes cell proliferation and infiltration, Colloids Surf. B Biointerfaces, 121 (2014) 432-443.

DOI: 10.1016/j.colsurfb.2014.06.034

[39] S.N. Hanumantharao, C. Que, S. Rao, Self-assembly of 3D nanostructures in electrospun polycaprolactone-polyaniline fibers and their application as scaffolds for tissue engineering, Materialia, 6 (2019) 100296.

DOI: 10.1016/j.mtla.2019.100296

[40] C. Yang, G. Deng, W. Chen, X. Ye, X. Mo, A novel electrospun-aligned nanoyarn-reinforced nanofibrous scaffold for tendon tissue engineering, Colloids Surf. B Biointerfaces, 122 (2014) 270-276.

DOI: 10.1016/j.colsurfb.2014.06.061

[41] D. Kai, M.P. Prabhakaran, B. Stahl, M. Eblenkamp, E. Wintermantel, S. Ramakrishna, Mechanical properties and in vitro behavior of nanofiber–hydrogel composites for tissue engineering applications, Nanotechnology, 23 (2012) 095705.

DOI: 10.1088/0957-4484/23/9/095705

[42] X.-Y. Dai, W. Nie, Y.-C. Wang, Y. Shen, Y. Li, S.-J. Gan, Electrospun emodin polyvinylpyrrolidone blended nanofibrous membrane: a novel medicated biomaterial for drug delivery and accelerated wound healing, J. Mater. Sci. Mater. Med., 23 (2012) 2709-2716.

DOI: 10.1007/s10856-012-4728-x

[43] J. Hu, M.P. Prabhakaran, L. Tian, X. Ding, S. Ramakrishna, Drug-loaded emulsion electrospun nanofibers: characterization, drug release and in vitro biocompatibility, RSC Advances, 5 (2015) 100256-100267.

DOI: 10.1039/c5ra18535a

[44] J.S. Choi, K.W. Leong, H.S. Yoo, In vivo wound healing of diabetic ulcers using electrospun nanofibers immobilized with human epidermal growth factor (EGF), Biomaterials, 29 (2008) 587-596.

DOI: 10.1016/j.biomaterials.2007.10.012

[45] I. Liao, S. Chew, K. Leong, Aligned core–shell nanofibers delivering bioactive proteins, (2006).

DOI: 10.2217/17435889.1.4.465

[46] H.R. Munj, J.J. Lannutti, D.L. Tomasko, Understanding drug release from PCL/gelatin electrospun blends, J. Biomater. Appl., 31 (2017) 933-949.

DOI: 10.1177/0885328216673555

[47] X. Yan, J. Marini, R. Mulligan, A. Deleault, U. Sharma, M.P. Brenner, et al., Slit-surface electrospinning: a novel process developed for high-throughput fabrication of core-sheath fibers, PLoS One, 10 (2015) 1-11.

DOI: 10.1371/journal.pone.0125407

[48] K. Wei, Y. Li, X. Lei, H. Yang, A. Teramoto, J. Yao, et al., Emulsion Electrospinning of a Collagen‐Like Protein/PLGA Fibrous Scaffold: Empirical Modeling and Preliminary Release Assessment of Encapsulated Protein, Macromolecular bioscience, 11 (2011) 1526-1536.

DOI: 10.1002/mabi.201100141

[49] L. Viry, S.E. Moulton, T. Romeo, C. Suhr, D. Mawad, M. Cook, et al., Emulsion-coaxial electrospinning: designing novel architectures for sustained release of highly soluble low molecular weight drugs, J. Mater. Chem., 22 (2012) 11347-11353.

DOI: 10.1039/c2jm31069d

[50] H.S. Yoo, T.G. Kim, T.G. Park, Surface-functionalized electrospun nanofibers for tissue engineering and drug delivery, Adv. Drug Deliv. Rev., 61 (2009) 1033-1042.

DOI: 10.1016/j.addr.2009.07.007

[51] K. Ye, H. Kuang, Z. You, Y. Morsi, X. Mo, Electrospun nanofibers for tissue engineering with drug loading and release, Pharmaceutics, 11 (2019) 182.

DOI: 10.3390/pharmaceutics11040182

[52] S. Jiang, B.C. Ma, W. Huang, A. Kaltbeitzel, G. Kizisavas, D. Crespy, et al., Visible light active nanofibrous membrane for antibacterial wound dressing, Nanoscale Horiz., 3 (2018) 439-446.

DOI: 10.1039/c8nh00021b

[53] A. Wang, C. Xu, C. Zhang, Y. Gan, B. Wang, Experimental investigation of the properties of electrospun nanofibers for potential medical application, J. Nanomater., 2015 (2015) 8 pages.

[54] J.S. Boateng, K.H. Matthews, H.N. Stevens, G.M. Eccleston, Wound healing dressings and drug delivery systems: a review, J. Pharm. Sci., 97 (2008) 2892-2923.

DOI: 10.1002/jps.21210

[55] Y. Zhang, C.T. Lim, S. Ramakrishna, Z.-M. Huang, Recent development of polymer nanofibers for biomedical and biotechnological applications, J. Mater. Sci. Mater. Med., 16 (2005) 933-946.

DOI: 10.1007/s10856-005-4428-x

[56] R.M. Abdel-Rahman, A. Abdel-Mohsen, R. Hrdina, L. Burgert, Z. Fohlerová, D. Pavliňák, et al., Wound dressing based on chitosan/hyaluronan/nonwoven fabrics: Preparation, characterization and medical applications, Int. J. Biol. Macromol., 89 (2016) 725-736.

DOI: 10.1016/j.ijbiomac.2016.04.087

[57] T. Sonia, C.P. Sharma, In vitro evaluation of N-(2-hydroxy) propyl-3-trimethyl ammonium chitosan for oral insulin delivery, Carbohydr. Polym., 84 (2011) 103-109.

DOI: 10.1016/j.carbpol.2010.10.070

[58] M.R. Ladd, S.J. Lee, J.D. Stitzel, A. Atala, J.J. Yoo, Co-electrospun dual scaffolding system with potential for muscle–tendon junction tissue engineering, Biomaterials, 32 (2011) 1549-1559.

DOI: 10.1016/j.biomaterials.2010.10.038

[59] N. Vitchuli, Q. Shi, J. Nowak, K. Kay, J.M. Caldwell, F. Breidt, et al., Multifunctional ZnO/Nylon 6 nanofiber mats by an electrospinning–electrospraying hybrid process for use in protective applications, Sci. Technol. Adv. Mat., 12 (2011) 055004.

DOI: 10.1088/1468-6996/12/5/055004

[60] S. Kidoaki, I.K. Kwon, T. Matsuda, Mesoscopic spatial designs of nano-and microfiber meshes for tissue-engineering matrix and scaffold based on newly devised multilayering and mixing electrospinning techniques, Biomaterials, 26 (2005) 37-46.

DOI: 10.1016/j.biomaterials.2004.01.063

[61] Y. Yokoyama, S. Hattori, C. Yoshikawa, Y. Yasuda, H. Koyama, T. Takato, et al., Novel wet electrospinning system for fabrication of spongiform nanofiber 3-dimensional fabric, Mater. Lett., 63 (2009) 754-756.

DOI: 10.1016/j.matlet.2008.12.042

[62] R.P. de Oliveira Santos, L.A. Ramos, E. Frollini, Cellulose and/or lignin in fiber-aligned electrospun PET mats: the influence on materials end-properties, Cellulose, 26 (2019) 617-630.

DOI: 10.1007/s10570-018-02234-7

[63] D. Li, Y. Wang, Y. Xia, Electrospinning of polymeric and ceramic nanofibers as uniaxially aligned arrays, Nano Lett., 3 (2003) 1167-1171.

DOI: 10.1021/nl0344256

[64] E. Zdraveva, B. Mijović, E. Govorčin Bajsić, I. Slivac, T. Holjevac Grgurić, A. Tomljenović, et al., Electrospun PCL/cefuroxime scaffolds with custom tailored topography, Journal of Experimental Nanoscience, 14 (2019) 41-55.

DOI: 10.1080/17458080.2019.1633465

[65] A.K. Higham, C. Tang, A.M. Landry, M.C. Pridgeon, E.M. Lee, A.L. Andrady, et al., Foam electrospinning: A multiple jet, needle‐less process for nanofiber production, AlChE J., 60 (2014) 1355-1364.

DOI: 10.1002/aic.14381

[66] M. Simonet, O.D. Schneider, P. Neuenschwander, W.J. Stark, Ultraporous 3D polymer meshes by low‐temperature electrospinning: use of ice crystals as a removable void template, Polym. Eng. Sci., 47 (2007) 2020-2026.

DOI: 10.1002/pen.20914

[67] P. Uttayarat, A. Perets, M. Li, P. Pimton, S.J. Stachelek, I. Alferiev, et al., Micropatterning of three-dimensional electrospun polyurethane vascular grafts, Acta Biomater., 6 (2010) 4229-4237.

DOI: 10.1016/j.actbio.2010.06.008

[68] J. Jiang, M.A. Carlson, M.J. Teusink, H. Wang, M.R. MacEwan, J. Xie, Expanding two-dimensional electrospun nanofiber membranes in the third dimension by a modified gas-foaming technique, ACS Biomater. Sci. Eng., 1 (2015) 991-1001.

DOI: 10.1021/acsbiomaterials.5b00238

[69] X. Liu, S. Liu, S. Liu, W. Cui, Evaluation of oriented electrospun fibers for periosteal flap regeneration in biomimetic triphasic osteochondral implant, J. Biomed. Mater. Res. B Appl. Biomater., 102 (2014) 1407-1414.

DOI: 10.1002/jbm.b.33119

[70] J. Nam, Y. Huang, S. Agarwal, J. Lannutti, Improved cellular infiltration in electrospun fiber via engineered porosity, Tissue Eng., 13 (2007) 2249-2257.

DOI: 10.1089/ten.2006.0306

[71] Y.H. Lee, J.H. Lee, I.-G. An, C. Kim, D.S. Lee, Y.K. Lee, et al., Electrospun dual-porosity structure and biodegradation morphology of Montmorillonite reinforced PLLA nanocomposite scaffolds, Biomaterials, 26 (2005) 3165-3172.

DOI: 10.1016/j.biomaterials.2004.08.018

[72] W. Zhu, N.J. Castro, X. Cheng, M. Keidar, L.G. Zhang, Cold atmospheric plasma modified electrospun scaffolds with embedded microspheres for improved cartilage regeneration, PLoS One, 10 (2015) 1-18.

DOI: 10.1371/journal.pone.0134729

[73] S. Zaiss, T.D. Brown, J.C. Reichert, A. Berner, Poly (ε-caprolactone) scaffolds fabricated by melt electrospinning for bone tissue engineering, Materials, 9 (2016) 232.

DOI: 10.3390/ma9040232