Bacterial Biofilm Development on Polyethylene with Organic and Inorganic Reagents In Vitro

Abstract:

The antimicrobial efficacy of polyethylene (PE) with organic antibacterial agent and inorganic antibacterial agent were evaluated in this work. Moreover, inhibition to bacterial biofilm on their surfaces was investigated in detail. Our experimental results showed that both modified PE samples exhibited excellent antimicrobial performances against S. aureus and E. coli with low cell suspension. When cell suspension increased up to10⁹ cell/ml, a large amount of bacteria (S. aureus and E. coli) and extracellular polysaccharide matrix adhered to the untreated PE and PE with inorganic antibacterial agent. On the other hand, adhesion, colonization and biofilm of S. aureus did not occur on PE with organic antibacterial agent, and a little E. coli survived on its surface. It was demonstrated that organic antibacterial agent had better ability to inhibit bacteria propagation than the inorganic one in initial time, and thus it prevented adherent bacteria to develop biofilm on the surface. The difference was derived from different initial effect time of them against bacteria. Therefore, it was a better approach to prevent catheter-related infections through addition of organic reagent into bulk material.

• Periodical: Journal of Biomimetics, Biomaterials and Tissue Engineering (Volume 16)
• Pages: 97-107
• DOI: https://doi.org/10.4028/www.scientific.net/JBBTE.16.97
• Online since: December 2012
• Authors: Hong Mei Li, Huan Xin Li, Wei Guo Zhao, Wei Zhang, Jun Hui Ji
• Keywords: Bacterial Inhabitation, Biofilm, Disinfection Adhesion

References

[1] Archibald LK. Gayness RP. Hospital acquired infections in the United States: the importance of interhospital comparisons. Infectious Disease Clinics of North America 1997; 11(2): 245-255.

DOI: 10.1016/s0891-5520(05)70354-8

[2] Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science; 1999; (284): 1318-1322.

DOI: 10.1126/science.284.5418.1318

[3] Nichols RL, Raad II. Management of bacterial complications in critically ill patients: surgical wound and catheter-related infections. Diagnose microbiology infectious disease 1999; 33(2): 121-130.

DOI: 10.1016/s0732-8893(98)00144-8

[4] Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. Lancet 2001; 358(9276): 135-138.

DOI: 10.1016/s0140-6736(01)05321-1

[5] Gristina AG, Hobgood CD, Webb LX. Adhesive colonization of biomaterials and antibiotic resistance. Biomaterials 1987; 8(6): 423-426.

DOI: 10.1016/0142-9612(87)90077-9

[6] Vuong C, Otto M. Staphylococcus epidermidis infections. Microbes and infection 2002; 4(4): 481-489.

DOI: 10.1016/s1286-4579(02)01563-0

[7] Fux CA, Costerton JW, Stewart PS et al. Survival strategies of infectious biofilms. Trends in Microbiology 2005; 13(1) , 34-40.

DOI: 10.1016/j.tim.2004.11.010

[8] Gerson DF, Scheer D. Cell surface energy, contact angle and phase partition III, Adhesion of bacterial cells to hydrophobic surfaces. Biochim. Biophys. Acta 1980; 602: 506-510.

DOI: 10.1016/0005-2736(80)90329-6

[9] Yu DG, Teng MY, Chou WL, Yang MC. Characterization and inhibitory effect of antibacterial PAN-based hollow fiber loaded with silver nitrate. Journal of Membrane Science 2003; 225: 115-123.

DOI: 10.1016/j.memsci.2003.08.010

[10] Zhang W, Chu PK, Ji JH . Antibacterial Properties of Plasma-Modified and Triclosan or Bronopol Coated Polyethylene. Polymer 2006; 47(3): 931-936.

DOI: 10.1016/j.polymer.2005.12.009

[11] Furno F, Morley KS, Wong B, et al. Silver nanoparticles and polymeric medical devices: a new approach to prevention of infection. Journal of Antimicrobial Chemotherapy 2004; 54(6): 1019-1024.

DOI: 10.1093/jac/dkh478

[12] Kalyon BD, Olgun U. Antibacterial efficacy of triclosan-incorporated polymers. American Journal of Infection Control. 2001; 29(2): 124-125.

DOI: 10.1067/mic.2001.113229

[13] Schweizer HP. Triclosan: a widely used biocide and its link to antibiotics. FEMS Microbiology Letters. 2001; 202(1-7): 1-7.

DOI: 10.1111/j.1574-6968.2001.tb10772.x

[14] Francolini I, Donelli G, Stoodley P. Polymer designs to control biofilm growth on medical devices. Rev Environ Sci Bio/Technol 2003; 2(2-4): 307-319.

DOI: 10.1023/b:resb.0000040469.26208.83

[15] Brady RA, Leid JG, Camper AK, Costerton JW, Shirtliff ME. Identification of Staphylococcus aureus Proteins Recognized by the Antibody-Mediated Immune Response to a Biofilm Infection. Infection and Immunity, 2006; 74(6): 3415-3426.

DOI: 10.1128/iai.00392-06

[16] Ji JH, Zhang W，Bacterial behaviors on polymer surfaces with organic and inorganic antimicrobial compounds. Journal of Biomedical Materials Research. 2009; 88A(2): 448-453.

DOI: 10.1002/jbm.a.31759

[17] Cook G, Costerton JW, Darouiche RO. Direct confocal microscopy studies of the bacterial colonization in vitro of a silver-coated heart valve sewing cuff. International Journal of Antimicrobial Agents. 2000; 13(3): 169-173.

DOI: 10.1016/s0924-8579(99)00120-x

[18] Braoudaki M, Hilton AC. Mechanisms of resistance in Salmonella enterica adapted to erythromycin, benzalkonium chloride and triclosan. International Journal of Antimicrobial Agents 2005; 25: 31-37.

DOI: 10.1016/j.ijantimicag.2004.07.016

[19] ISO 21996-2007(E) : Plastics Measurement of antibacterial activity on plastics surfaces.

[20] Hope CK, Wilson M. Biofilm structure and cell vitality in a laboratory model of subgingival plaque. Journal of Microbiological Methods 2006; 66(3): 390-398.

DOI: 10.1016/j.mimet.2006.01.003

[21] Tatar EC, Unal FO, Tatar I, et al. Investigation of surface changes in different types of ventilation tubes using scanning electron microscopy and correlation of findings with clinical follow-up. International Journal of Pediatric Otorhinolaryngology 2006; 70(3): 411-417.

DOI: 10.1016/j.ijporl.2005.07.005

[22] Little B, Wagner P, Ray R, Pope R, Scheetz R, Biofilms: An ESEM evaluation of artifacts introduced during SEM preparation. Journal of Industrial Microbiology and Biotechnology 1991; 8( 4): 213-221.

DOI: 10.1007/bf01576058

[23] Hope CK, Wilson M. Measuring the thickness of an outer layer of viable bacteria in an oral biofilm by viability mapping. Journal of Microbiological Methods 2003; 54: 403-410.

DOI: 10.1016/s0167-7012(03)00085-x

[24] Jefferson KK. What drives bacteria to produce a biofilm. FEMS Microbiology Letters 2004; 236: 163-173.

DOI: 10.1111/j.1574-6968.2004.tb09643.x

[25] Valle J, Toledo-Arana A, Berasain C, Ghigo JM, Amorena B, Penades JR, Lasa, I. SarA and not sigma(B) is essential for biofilm development by Staphylococcus aureus. Molecular Microbiology. 2003; 48: 1075-1087.

DOI: 10.1046/j.1365-2958.2003.03493.x

[26] Corona-Izquierdo FP. Membrillo-Hernandez J. A mutation in rpoS enhances biofilm formation in Escherichia coli during exponential phase of growth. FEMS Microbiology Letters. 2002; 211: 105-110.

DOI: 10.1111/j.1574-6968.2002.tb11210.x

[27] Shapiro JA. Thinking about bacterial populations as multicellular organisms. Annual Review of Microbiology 1998; 52: 81-104.

DOI: 10.1146/annurev.micro.52.1.81

[28] Federle MJ, Bassler BL. Interspecies communication in bacteria. Journal of Clinical Investigation 2003; 112: 1291-1299.

DOI: 10.1172/jci20195

[29] Kong KF, Vuong C, Otto M, Staphylococcus quorum sensing in biofilm formation and infection, International Journal of Medical Microbiology, 2006; 296( 2), 133-139.

DOI: 10.1016/j.ijmm.2006.01.042

[30] Li MY, Zhang J, Lu P, Xu JL，Li SP. Evaluation of Biological Characteristics of Bacteria Contributing to Biofilm Formation. Pedosphere 2009; 19(5): 554-561.

DOI: 10.1016/s1002-0160(09)60149-1