The Preparation of Silk Fibroin Modified PBT-co-PBS/PEG Composite Films and their Effects on Clinical Human Salivary Epithelial Cells Transplantation

Abstract:

We reported the preparation of surface modified poly(butylene terephthalate)-co-poly(butylene succinate)-b-poly(ethylene glycol) (i.e. PBT-co-PBS/PEG) films by three methods: silk fibroin coating, SO₂ plasma treatment and silk fibroin anchoring. The obtained composite films were named SF/(PBT-co-PBS/PEG), SO₂/(PBT-co-PBS/PEG) and SF/SO₂/(PBT-co-PBS/PEG), respectively. Their surface properties were characterized by contact angles, surface energies and XPS. The biocompatibility of the films were further evaluated by the morphology, attachment, proliferation and viability of human salivary epithelial cells (HSG cells). Results revealed that SF/SO₂/(PBT-co-PBS/PEG) possessed the high surface free energy (59.67 mJ/m²) and could immobilize a great amount of fibroin (SF surface coverage: 26.39 wt%), which attributed to the formation of such polar groups as hydrosulfide group, sulfonic group, carboxyl and carbonyl ones in the process of SO₂ plasma treatment. The cell tests suggested that the silk fibroin anchoring could significantly enhance the biocompatibility of PBT-co-PBS/PEG, which implied the potential application of fibroin modified PBT-co-PBS/PEG for clinical HSG cells transplantation in artificial salivary gland constructs.

• Periodical: Journal of Biomimetics, Biomaterials and Tissue Engineering (Volume 18)
• Pages: 29-42
• DOI: https://doi.org/10.4028/www.scientific.net/JBBTE.18.29
• Online since: December 2013
• Authors: Jie Zhu*, Yue Ming Zhang, Zhi Cheng Tao, Nan Wei Xu, Li Qun Wang, Xiao Lin Zhu
• Keywords: Biocompatibility, Human Salivary Epithelial Cell Line, Poly(Butyleneterephthalate)-co-Poly(Butylenesuccinate)-b-Poly(Ethylene Glycol), SO₂ Anchoring, Surface Modification

References

[1] T. Sugihara and Y. Yoshimura: Inter. J. Oral Maxillofac. Surg. Vol. 17 (1988), p.71

[2] L. Sabrina, S. Margherita and L. Domenico: Lab Investig. Vol. 92 (2012), p.615

[3] J. Meredith, Y. Eugene and K. John: Expert Rev. Anticancer Ther. Vol. 12 (2012), p.359

[4] S.L. Wang, E. Cukierman, W.D. Swaim, K.M. Yamada and B.J. Baum: Biomaterials Vol. 20 (1999), p.1043

[5] J.P. Chen and C.H. Su: Acta. Biomater. Vol. 7 (2011), p.234

[6] R. Jean-Gilles, D. Soscia, S.J. Sequeira, M. Melfi and A. Gadre: J. Nanotechnol. Eng. Med. Vol. 1 (2010), p.31008

[7] S. Sequeira, D. Soscia, B. Oztan, A. Mosier and R. Jean-Gilles: Biomaterials Vol. 23 (2012), p.3175

[8] U. Witt, R.J. Müller and W.D. Deckwer: Macromol. Chem. Phys. Vol. 197 (1996), p.1525

[9] R.J. Müller, I. Kleeberg and W.D. Deckwer: J. Biotech. Vol. 86 (2001), p.87

[10] A.E. Ghalbzouri, E.N. Lamme, C. Van Blitterswijk, J. Koopmanc and M. Ponec: Biomaterials Vol. 25 (2004), p.2987

DOI: 10.1016/j.biomaterials.2003.09.098

[11] J. Malda, T.B.F. Woodfield, F. Van de Vloodt, C.D.E. Wilson Martens and J. Tramper: Biomaterials Vol. 26 (2005), p.63

DOI: 10.1016/j.biomaterials.2004.02.046

[12] S. L. Luo, F. X. Li and J. Y. Yu: J. Polym. Res. Vol. 18 (2011), p.393

[13] Y. Zhang, Z.G. Feng, F.X. Liu and A.Y. Zhang: Acta. Chimica. Sinica. Vol. 60 (2002), p.2225

[14] A.O. Lobo, F.R. Marciano, S.C. Ramos, M.M. Machado and E.J. Corat: Mater. Sci. Eng. C Vol. 31 (2011), p.1505

[15] L. Moroni, R. Licht, J. Boer, J.R. Wijn and C.A. Van Blitterswijk: Biomaterials Vol. 27 (2006), p.4911

[16] Y. L. Cui, A. D. Qi, W. G. Liu, X. H. Wang, H. Wang, D. M. Ma and K. D. Yao: Biomaterials Vol. 24 (2003), p.3859

[17] Z. Ma, C. Gao, Y. Gong and J. Shen: Biomaterials Vol. 26 (2005), p.1253

[18] R.F. She, J. Deng, W.L. Huang, Z.J. Dong and B. Liu: Chin. J. Tissue Eng. Res. Vol. 16 (2012), p.455

[19] Q. Wang and L. Qi: New Chem. Mater. Vol. 40 (2012), p.76

[20] D.G. Harkin, K.A. George, P.W. Madden, I.R. Schwab and D.W. Hutmacher: Biomaterials Vol. 32 (2011), p.2445

[21] A.S. Gobin, V.E. Froude and A.B. Mathur: J. Biomed. Mater. Res. Vol. 74 (2005), p.465

[22] C.C. Hou, S.J. Tao, Y. Zhu and S. Xu: J. Text. Res. Vol. 33 (2012), p.27

[23] P. Madden, J.N.X. Lai, K. George, T. Giovenco and D. Harkin: Biomaterials Vol. 32 (2011), p.4076

[24] K.Y. Cai, K.D. Yao, Y.L. Cui, Z.M. Yang and X.Q. Li : Biomaterials Vol. 23 (2002), p.1603

[25] X.F. Wang, B. Ding, J.Y. Yu and M.R. Wang: Nano today Vol. 6 (2011), p.510

[26] Z. Ding, J.N. Chen, S.Y. Gao, J.B. Chang and J.F. Zhang: Biomaterials Vol. 25 (2004), p.1059

[27] A.E. Ghalbzouri, E.N. Lamme, C. Van Blitterswijk, J. Koopmanc and M. Ponec: Biomaterials Vol. 25 (2004), p.2987

DOI: 10.1016/j.biomaterials.2003.09.098

[28] J. Malda, T.B.F. Woodfield, F. Van de Vloodt, C.D.E. Wilson Martens and J. Tramper: Biomaterials Vol. 26 (2005), p.63

DOI: 10.1016/j.biomaterials.2004.02.046

[29] D.K. Owens and R.C. Wendt: J. Appl. Polymer Sci. Vol. 13 (1969), p.1741

[30] Z.F. Su and B.Q. Zuo: Silk Vol. 48 (2011), p.13

[31] K. Inuvye, M. Kurokawa, S. Nishikawa and M. Tsukada: J. Biochem. Biophys. Methods Vol. 37 (1998), p.159