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A Facile Synthesis of Acid-Activatable Nanoparticles for Efficient Intracellular Doxorubicin Release

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Abstract:

pH responsive polymeric nanoparticles have emerged as a promising technology platform for targeted and controlled drug delivery in recent years. In this paper, endosomal pH-activatable doxorubicin (DOX) and core-crosslinked polymeric nanoparticles (DCNPs) were prepared and investigated for potent growth inhibition of human cancer cells in vitro. In vitro drug release studies, DOX conjugated nanoparticles with hydrazone bond showed a pH sensitive release phenomenon, that is, the releasing is significantly faster at mildly acidic condition with pH of 5.5 than that at physiological condition. Confocal laser scanning microscope (CLSM) observations revealed that DOX conjugated nanoparticles delivered and released DOX into the cytosols as well as cell nuclei of Hela cells following 6 h incubation. MTT assays demonstrated that these pH-sensitive DOX nanoparticles exhibited high antitumor effect to HeLa cells. The conjugated DOX polymeric nanoparticles may be a promising candidate as a nanoscale and pH-sensitive drug delivery vehicle for cancer therapy.

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Journal of Nano Research Vol. 46 View Preview

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Periodical:

Journal of Nano Research (Volume 46)

Pages:

20-30

DOI:

https://doi.org/10.4028/www.scientific.net/JNanoR.46.20

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Online since:

March 2017

Authors:

Cao Ming*, Xiao Wan Song, Yu Jiao Zhang, Chang Zhi Xu, Peng Chen, Ru Xia, Tian Long Hu, Jia Sheng Qian, Ke Liang Wu

Keywords:

Doxorubicin, Drug Delivery, Intracellular Drug Release, Nanoparticles, pH-Sensitive

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© 2017 Trans Tech Publications Ltd. All Rights Reserved

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