Effects of Electromagnetic Fields Exposure on the Production of Nanosized Magnetosome, Elimination of Free Radicals and Antioxidant Defense Systems in Magnetospirillum gryphiswaldense MSR-1

Abstract:

Magnetotactic bacteria integrated magnetosomes, which are unique organelles that contain nanosized crystals of biogenic magnetic iron minerals with the ability to respond to the external magnetic fields. The biogenic magnetic nanoparticles (magnetosomes) show high biocompatibility in medical applications especially as scavengers to eliminate intracellular reactive oxygen species. The aim of this study was to highlight the impact of magnetosome formation and antioxidant systems in the suppression of oxidative stress on the magnetotactic bacteria cells. To assess the changes in ROS levels under different magnetic field intensity conditions, cells were cultured under the microaerobic condition in medium containing the high and low intensity of magnetic field. Treatment of magnetic field with an intensity of 500 mT during 50 hours bionormalization process of magnetotactic bacteria increased the antioxidant enzyme activity for eliminating of free radicals by 64%. We concluded that magnetosomes production plays an important role in decreasing or eliminating ROS. This is the first study to demonstrate that the magnetic field assisted magnetosome formation and antioxidants defense systems in Magnetospirillum gryphiswaldense MSR-1.

• Periodical: Journal of Nano Research (Volume 58)
• Pages: 20-31
• DOI: https://doi.org/10.4028/www.scientific.net/JNanoR.58.20
• Online since: June 2019
• Authors: Hatami Giklou Jajan Leila, Mohsen Abolhassani*, Seyed Nezamedin Hosseini, Behzad Ghareyazie, Leila Ma'mani, Delaram Doroud, Ava Behrouzi, Masoud Ghorbani
• Keywords: Antioxidant Enzymes Activity, Elimination Of ROS, M. gryphiswaldense MSR-1, Magnetic Field, Magnetosome

References

[1] D.A. Bazylinski, R.B. Frankel, Nat. Rev. Microbiol. 2 (2004) 217.

[2] C.T. Lefèvre, D.A. Bazylinski, Microbiol. Mol. Biol. Rev. 77 (2013) 497 LP-526.

[3] F. Fang Guo, W. Yang, W. Jiang, S. Geng, T. Peng, J. Lun Li, Magnetosomes Eliminate Intracellular Reactive Oxygen Species in Magnetospirillum Gryphiswaldense MSR‐1, (2012).

DOI: 10.1111/j.1462-2920.2012.02707.x

[4] S.S. Staniland, A.E. Rawlings, Biochem. Soc. Trans. 44 (2016) 883–890.

[5] A. Arakaki, H. Nakazawa, M. Nemoto, T. Mori, T. Matsunaga, J. R. Soc. Interface 5 (2008) 977–999.

[6] E. Alphandéry, Front. Bioeng. Biotechnol. 2 (2014) 5.

[7] C. Moisescu, I.I. Ardelean, L.G. Benning, Front. Microbiol. 5 (2014) 49.

[8] F.F. Guo, W. Yang, W. Jiang, S. Geng, T. Peng, J.L. Li, Environ. Microbiol. 14 (2012) 1722–1729.

[9] F.F. Guo, W. Yang, W. Jiang, S. Geng, T. Peng, J.L. Li, G.F. Fang, Y. Wei, J. Wei, G. Shuang, P. Tao, L.J. Lun, Environ. Microbiol. 14 (2012) 1722–1729.

[10] F.F. Guo, W. Yang, W. Jiang, S. Geng, T. Peng, J.L. Li, Environ. Microbiol. 14 (2012) 1722–1729.

[11] M.E. Maffei, Front. Plant Sci. 5 (2014) 445.

[12] Z. Ren, X. Leng, Q. Liu, Water Sci. Technol. (2017).

[13] Y. Wang, W. Lin, J. Li, Y. Pan, Front. Microbiol. 4 (2013).

[14] H. Wang, X. Zhang, Int. J. Mol. Sci. 18 (2017) 1–20.

[15] C. Dahmani, Static and Dynamic Magnetic Fields for the Nanoparticle Based Site-Directed Drug Delivery, (2014).

[16] R. Popa, W. Fang, K.H. Nealson, V. Souza-Egipsy, T.S. Berqú, S.K. Banerje, L.R. Penn, Int. Microbiol. 12 (2009) 49–57.

[17] E.G. Kıvrak, K.K. Yurt, A.A. Kaplan, I. Alkan, G. Altun, J. Microsc. Ultrastruct. 5 (2017) 167–176.

[18] M. Domenech, I. Marrero-Berrios, M. Torres-Lugo, C. Rinaldi, ACS Nano 7 (2013) 5091–5101.

DOI: 10.1021/nn4007048

[19] V.N. Binhi, F.S. Prato, Biological Effects of the Hypomagnetic Field: An Analytical Review of Experiments and Theories, (2017).

DOI: 10.1371/journal.pone.0179340

[20] A. (Scenihr), Opin. Sci. Comm. EU (2009).

[21] M. Circu, T.Y. Aw, Free Radic Biol Med. 2010 48 (2010) 749–762.

[22] M.-O. Mattsson, M. Simkó, Front. Public Heal. 2 (2014) 132.

[23] M. Simko, Cell Type Specific Redox Status Is Responsible for Diverse Electromagnetic Field Effects, (2007).

DOI: 10.2174/092986707780362835

[24] J.-B. Sun, F. Zhao, T. Tang, W. Jiang, J. Tian, Y. Li, J.-L. Li, Appl. Microbiol. Biotechnol. 79 (2008) 389.

[25] Y. Liu, G.R. Li, F.F. Guo, W. Jiang, Y. Li, L.J. Li, Microb. Cell Fact. 9 (2010) 99.

[26] D. Schüler, E. Baeuerlein, J. Bacteriol. 180 (1998) 159–162.

[27] F. Fang, Y. Li, G.-C. Du, J. Zhang, J. Chen, Sheng Wu Gong Cheng Xue Bao 20 (2004) 423–428.

[28] H.U. Bergmeyer, J. Bergmeyer, M. Grassl, Methods of Enzymatic Analysis, Verlag Chemie, Weinheim; Deerfield Beach, Fla., (1983).

DOI: 10.1016/0014-5793(84)81399-x

[29] C. Yingqing, L. Zhongxiong, S. Jinshan, C. Yiting, L. Guoqing, L. Yuling, Z. Wenlu, CHINESE J. Trop. Crop. 27 (2006) 29–33.

[30] D. Schüler, R. Uhl, E. Bäuerlein, FEMS Microbiol. Lett. 132 (1995) 139–145.

[31] L. Zhao, D. Wu, L.-F. Wu, T. Song, J. Biochem. Biophys. Methods 70 (2007) 377–383.

[32] (n.d.).

[33] Ö. Çelik, N. Büyükuslu, Ç. Atak, A. Rzakoulieva, Pol J Env. Stud 18 (2009) 175–182.

[34] J. Li, Y. Yi, X. Cheng, D. Zhang, M. Irfan, Bot. Stud. 56 (2015) 1–20.

[35] J. Li, Y. Yi, X. Cheng, D. Zhang, M. Irfan, Bot. Stud. 56 (2015) 2.