Spectroscopy Resonance Plasmon Efficient Tool for Cell Adsorption

Abstract:

It is important to analyze cell monolayer adherence for the development of biomedical devices of anti-thrombogenic vascular grafts. Endothelial cells must be firmly attached to the biomaterials when cells are seeded in order to create a natural lining. Polystyrene (PS) is presented as a reproducible implant model substrate for studying cell – material interactions. Polystyrene was deposited as a thin layer on a thiol functionalized gold electrode. Fibronectin (Fn), a protein promoting the cell monolayer adhesion was adsorbed on PS surface. The different steps of this multilayer assembly were characterized by Surface Plasmon Resonance (SPR) technique. A right shift of the SPR resonance angle θ_SPR was observed leading an increase from 65.5 deg in the case of gold electrode to 66.8 deg in the case where cell monolayer was cultured onto functionalized gold substrate. A shift in the SPR peak minimum intensity was detected in the SPR response of Au/Thiol/PS/Fn and Au/Thiol/PS/Fn/Cell multilayer assembly structures. This result is explained using Atomic Force Microscopy (AFM) images and according transverse profiles which indicate surface morphological modifications in term of thickness.

• Periodical: Journal of Nano Research (Volume 59)
• Pages: 35-45
• DOI: https://doi.org/10.4028/www.scientific.net/JNanoR.59.35
• Online since: August 2019
• Authors: Amira Bouafsoun*, Nicole Jaffrezic-Renault, Laurence Mora
• Keywords: Cell Adsorption, Cell Monolayer, Functionalized Substrates, Morphology, Resonance Angle, SPR Response

References

[1] Wood. R.W., Philos. Mag. Ser. , 6 (4) (1902) 396 – 402.

[2] Liedberg B. Nylander C. Lunstrom I. Sens. Actuators B 4 ( 1983) 299 – 304.

[3] Scarano, S.; Mascini, M.; Turner, A.P.F.; Minunni, M. Surface plasmon resonance imaging for affinity-based biosensors. Biosens. Bioelectron. 25 (2010) 957–966.

DOI: 10.1016/j.bios.2009.08.039

[4] Cooper, M.A. Lable-free screening of bio-molecular interactions. Anal. Bioanal. Chem. 377 (2003) 834–842.

[5] Clark, L.C.; Lyons, C. Electrode systems for continuous monitoring in cardiovascular surgery. Ann. N.Y. Acad. Sci. 102 (1962) 29–45.

[6] Yao, X.; Li, X.; Toledo, F.; Zurita-Lopez, C.; Gutova, M.; Momand, J.; Zhou, F. Sub-attomole oligonucleotide and p.53 cDNA determinations via a high-resolution surface plasmon resonance combined with oligonucleotide-capped gold nanoparticle signal amplification. Anal. Biochem. 354 (2006) 220–228.

DOI: 10.1016/j.ab.2006.04.011

[7] Yanase, Y., Suzuki, H., Tsutsui, T., Hiragun, T., Kameyoshi, Y., Hide, M. Biosens. Bioelectron. 22 (2007) 1081-1086.

[8] Homola, J.; Yee, S.S.; Gauglitz, G. Surface plasmon resonance sensors: Review. Sens. Actuators B Chem. 54 (1999) 3–15.

DOI: 10.1016/s0925-4005(98)00321-9

[9] H. Raether, in: G. Hass, M. Francombe, R. Hoffman (Eds). Physics of Thin Films, Academic Press, New-York 1977 p.145 – 261.

[10] A. Szabo, L. Stolz, R. Granow, Curr. Opin. Struct. Bio, 5 (1995) 699 – 705.

[11] E. Ostuni, L.Yan, G.M. whitesides, Colloids and surfaces B. Biointerfaces 15 (1993) 3 – 30.

[12] E. Ostuni, L.Yan, G.M. whitesides, Colloids and surfaces B. Biointerfaces 15 (1993) 3 – 30.

[13] H. J. Kim, I. T. Chang, S. J. Heo, J. Y. Koak, S. K. Kim, J. H. Jang, Clinical Oral Implants Research, 16 (2005) 557.

[14] G. Altankov, V. Thom, T. Groth, K. Jankova, G. Jonsson, M. Ulbrich, Journal of Biomedical Materials Research, 52 (2000) 219.

[15] J. Hoddle, R. Record, R. Tullius, S. Badylak, Biomaterials, 23 (2002) 1841.

[16] A. Bouafsoun, A. Othmane, N. Jaffrezic-Renault, A. Kerkeni, O. Thoumire, A.F. Prigent and L. Ponsonnet, Materials Science and Engineering C 28 (2008) 653-661.

DOI: 10.1016/j.msec.2007.10.073

[17] T. Viitala, N. Granqvist, S. Hallila, M. Ravina, M. Yliperttula, Plos ONE 8 (8) 2013, e 72192,.

[18] V.I. Chegel, Yu. M. Shirshov, sensor Electronics and Microsystems Technologies 2 (2004) 34 – 49.