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Photoregulation of Biosynthetic Activity of Lentinula edodes (Berk.) Pegler Using Colloidal Solutions of Metal Nanoparticles and Low-Intensity Laser Radiation

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Abstract:

The paper presents new data on the complex use of colloidal solutions of nanoparticles (NPs) and low-intensity laser radiation on the biosynthetic activity of the edible medicinal mushroom Lentinula edodes (L. edodes) in vitro. Traditional mycological methods, colloidal solutions of metals (Ag, Fe, and Mg NPs), and unique photobiological methods were used. An argon laser at wavelength of 488 nm was used as a source of coherent visible light. It was found that colloidal solutions of NPs of all used metals increased (26–39%) the growth characteristics of L. edodes, while irradiation of the fungus inoculum with laser light in a medium with NPs reduced the growth activity of the L. edodes mycelium. The addition of all NPs to the nutrient medium with the inoculum inhibited the synthesis of extracellular polysaccharides, the greatest effect was observed with Fe NPs. At the same time, laser light irradiation in the presence of NPs increased the amount of extracellular polysaccharides; the greatest effect was observed in photoinduced Mg NPs, which stimulated the synthesis of extracellular polysaccharides by 47%. The introduction of NPs into the inoculum reduced the amount of intracellular polysaccharides in the mycelial mass; the greatest inhibitory effect of 50% was observed for Ag NPs. At for the photoinduced NPs, they stimulated the synthesis of intracellular polysaccharides in the mycelial mass of L. edodes. Treatment of the inoculum in a medium with NPs and photoinduced NPs caused an intensification of the synthesis of phenolic compounds in the mycelial mass and an increase in radical scavenging activity (RSA). The highest RSA values were recorded for samples obtained from inoculum treatment with photoinduced Fe and Mg NPs, respectively, for methanol and ethanol extracts of mycelial mass. The obtained results suggest the possibility of complex use of colloidal solutions of Fe, Ag, and Mg NPs and low-intensity laser radiation as environmentally friendly factors regulating biosynthetic activity in biotechnology of cultivating the valuable medicinal fungus L. edodes.

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Periodical:

Key Engineering Materials (Volume 1028)

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105-120

DOI:

https://doi.org/10.4028/p-gHAqt1

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Online since:

October 2025

Authors:

Oksana Mykchaylova*, Anatoliy Negriyko, Konstantin Lopatko, Natalia Poyedinok

Keywords:

Antioxidant Activity, In Vitro Study, Laser, Metal Nanoparticles, Mycelial Biomass, Polysaccharides, Total Phenol Compounds

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