Production of Novel Exopolysaccharide with Emulsifying Ability from Marine Microorganism, Alteromonas sp. Strain 00SS11568

Abstract:

To find a novel exopolysaccharide, marine bacterial strains were isolated from coastal regions of Korea. Strain 00SS11568 was then selected as it produced a mucous exopolysaccharide during the stationary phase in a batch culture. The isolate was identified as Alteromonas sp. based on its 16S rDNA sequence, morphological, and biochemical properties. The exopolysaccharide, designated as p-11568, exhibited an emulsifying ability. The Emulsification Index (E24) of 0.1% p- 11568 was 77.4% with an emulsified kerosene content, and was higher than those of commercial polysaccharides, such as xanthan gum (26.1%), gellan gum (1.3%), and sodium alginate (2.0%). p- 11568 was found to be composed of glucose and galactose as the main natural sugars in a molar ratio of 1.3:1, along with uronic acid (18.9%, w/w) and sulfate groups (1.2% w/w). The average molecular mass was 4.4 x 105 daltons by gel filtration chromatography. The effects of pH, temperature, inorganic compounds, and C and N sources were tested to obtain the optimal medium composition for the production of p-11568. Under optimal growth conditions with the M-11568 medium, 14.9 g of crude p-11568 per liter was obtained.

• Periodical: Key Engineering Materials (Volumes 277-279)
• Pages: 155-161
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.277-279.155
• Online since: January 2005
• Authors: Joung Han Yim, Se Hun Ahn, Sung Jin Kim, Yoo Kyung Lee, Kyu Jin Park, Hong Kum Lee
• Keywords: Alteromonas, Culture Condition, Emulsifier, Exopolysaccharide, Marine Bacteria

References

[1] Becher P.: (1988) Encyclopedia of Emulsion Technology, vol. 3. Marcel Dekker, New York.

[2] Banat, I., R.S. Makkar and S.S. Cameotra: Appl. Microbiol. Biotechnol. (2000) 53: 495-508.

[3] Rosenberg, E. and E.Z. Ron: Appl. Microbiol. Biotechnol. (1999) 52: 154-162.

[4] Cirigliano, M.C. and G.M. Carman: Appl. Environ. Microbiol. (1985) 50: 846-850.

[5] Fiechter, A.: Trend Biotechnol. (1992) 10: 208-217.

[6] Matsuda, M. and W. Worawattanamateekul: Nippon Suisan Gakkaishi. (1993) 59: 875-878.

DOI: 10.2331/suisan.59.875

[7] Philippis, R., M.C. Margheri, E. Pelosi and S. Ventura: J. Appl. Phycol. (1993) 5: 387-394.

[8] Raguenes, G., P. Pignet, G. Gauthier, A. Peres, R. Christen, H. Rougeaux, G. Barbier and J. Guezennec: . Appl. Environ. Microbiol. (1996) 62: 67-73. Title of Publication (to be inserted by the publisher).

DOI: 10.1128/aem.62.1.67-73.1996

[9] Rodrigues, C. and N.B. Bhosle: Biofouling (1991) 4: 301-308.

[10] Maidak, B.L., J.R. Cole, C.T. Parker, G.M. Garrity Jr., N. Larsen, B. Li, T.G. Lilburn, M.J. McCaughey, G.J. Olsen, R. Overbeek, S. Pramanik, T.M. Schmidt, J,.M. . Tiedje and C.R. Woese: Nucleic Acids Res. (1999) 27: 171-173.

DOI: 10.1093/nar/27.1.171

[11] Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D. Lipman: Nucleic Acids Res. (1997) 53: 3389-3402.

[12] Brown M,J. and J.M. Lester: Appl. Environ. Microbiol. (1980) 40: 179-185.

[13] Miller, G.L.: Anal. Chem. (1959) 31: 426-428.

[14] Cameron, D.R., D.G. Cooper and R.J. Neufeld: Appl. Environ. Microbiol. (1988) 54: 1420-1425.

[15] Iwamuro, Y., M. Murata, K. Kanamuru, Y. Mikami and T. Kisaki: Agri. Biol. Chem. (1981) 45: 635-637.

[16] Darling, D.F. and R.J. Birkett: In E. Dickinson (Ed. ), Food Emulsions and Foams Royal Society of Chemistry, London. (1987) p, 1-29.

[17] Prud'homme, R. K, R.E. Long: J. Colloid. Interf. Sci., (1983) 93: 274-276.