In Situ Monitoring of Chondrocyte Response to Bioactive Scaffolds Using Raman Spectroscopy

Julian R. Jones; Archana Vats; Ioan Notingher; Julie E. Gough; Neil S. Tolley; Julia M. Polak; Larry L. Hench

doi:10.4028/www.scientific.net/KEM.284-286.623

Paper Titles

Development of Controlled Heterogeneity on a Polymer-Ceramic Hydrogel Scaffold for Osteochondral Repair
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Apatite Layer with Serum Protein as a Suitable Scaffold for Growth of Osteoblast-Like Cell
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Bisphosphonates Incorporated on a Ca-P Biomimetic Coating Produced by a Sodium Silicate Based Methodology Stimulate Osteoblastic Activity
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Solution Mediated Effect of Bioactive Glass in Poly (Lactic-Co-Glycolic Acid)- Bioactive Glass Composites on Osteogenesis of Marrow Stromal Cells
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In Situ Monitoring of Chondrocyte Response to Bioactive Scaffolds Using Raman Spectroscopy
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Incorporation of Human Osteoblast Cells and Osteoblast-Like Cells into Porous Hydroxyapatite Scaffolds
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Influence of Emdogain^® Treatment onto K₂O Incorporated Calcium Metaphosphate on Osteogenic Activation
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Evaluation of Osteoblast Viability, Alkaline Phosphatase Production and Collagen Secretion in the Presence of Hydroxyapatite Reinforced with Oxide Glasses
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Biocompatibility Evaluation of Three Different Titanium-Hydroxyapatite Composites
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HomeKey Engineering MaterialsKey Engineering Materials Vols. 284-286In Situ Monitoring of Chondrocyte Response to...

In Situ Monitoring of Chondrocyte Response to Bioactive Scaffolds Using Raman Spectroscopy

Abstract:

Septal cartilage is widely used for the repair of soft tissue defects in the head, neck and nose. Tissue Engineering techniques are being investigated to create cartilage in vitro by seeding appropriate cells on resorbable scaffolds. In this study, human chondrocytes were cultured on macroporous bioactive glass foam scaffolds. The aim was to investigate how Raman spectroscopy could be used as a non-invasive technique to monitor the response of chondrocytes to a 3D scaffold in real time. The spectra were compared to scanning electron microscope (SEM) micrographs and immunohistochemistry results.

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Periodical:

Key Engineering Materials (Volumes 284-286)

Pages:

623-626

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.284-286.623

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Online since:

April 2005

Authors:

Julian R. Jones, Archana Vats, Ioan Notingher, Julie E. Gough, Neil S. Tolley, Julia M. Polak, Larry L. Hench

Keywords:

Bioactive Glass, Cartilage, Chondrocytes, Raman Spectroscopy, Scaffold

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